家蚕滞育卵长期保存的研究

调查了不同温度保存的９个原种及２对杂交种的滞育性及多元醇量的变化，对长期保存卵进行了养蚕试验。结果表明：５℃保存卵在产卵１００日后滞育开始解除，１５０日左右孵化率达到最高峰，２００日后急剧下降。不同品种间无明显差异。１℃保存卵在产卵后１８０日左右开始解除滞育，原种在２００－３００日之间孵化率最高，３００日后显著降低，而杂交种产卵后４５０日孵化率仍在８０％以上。－１．５℃保存卵在产卵后５００日仍呈滞育状态，但转入５℃保存８０日后可获得孵化能力，到产卵后７００日孵化率大致在６０％左右。不同温度保存卵滞育的解除，与卵内山梨醇及甘油量的减少呈相同趋势。养蚕结果表明，用１℃保存４１０日以及－１．５℃保存７００日的杂交种，除孵化率比当年生产的冷藏浸酸种低外，其它饲育成绩无明显差异。

STUDIEA ON LONG-TERM PRESERVATION OF SILKWORM DIAPUSE EGGS

The dispause and concentration change of sorbitiol,glycerol of 9 parent races and 2hybrids being kept at different temperatures ,and the rearing experlments of the eggswith long-term preservation were studie.The results showed that the eggs being keptat 5℃ for 100 days after oviposition began to remove termination of diapause , but nosignificant difference among varieties. The eggs began to remove termination of dia-pause after they were preserved at 1℃ for about 180 days. The hatchability of parenteggs decreased sharply after 300 days, but that of the hybrids still kept over 80%at 1℃for 450 days after oviposition,The eggs being kept at-1.5℃ for 500 days was stiil indiapause , but the eggs can get the hatchability when they were transfered to 5℃ and in-cubated for 80 days,the hatchability was about 60%at 700 days. There were the sametendency between termination of the eggs diapause at different temperatures and sor-bitol and glyeorols deerease in the eggs. According to the rearing experiments , all therearing achievements of hybrids being kept at -1.5℃ for 700 days have no significantdifference, except the hatchability was lower than the eggs by acid treatment ater refrig-eration.