| 转柞蚕抗菌肽D基因辣椒的目的基因及表达产物检测 |
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| 引用本文： | 廖富蘋,钟杨生,邓平建,黄自然,刘建军,房师松,赵锦,陈迎. 转柞蚕抗菌肽D基因辣椒的目的基因及表达产物检测[J]. 蚕业科学, 2005, 31(2): 155-160 |
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| 作者姓名： | 廖富蘋 钟杨生 邓平建 黄自然 刘建军 房师松 赵锦 陈迎 |
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| 作者单位： | 华南农业大学动物科学学院蚕丝科学系,广州,510642;深圳市疾病预防控制中心,深圳,518020 |
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| 基金项目： | 国家自然科学基金 , 广东省科技厅科技计划 |
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| 摘 要： | 应用现代生物技术,从核酸和蛋白质水平对转柞蚕抗菌肽D基因辣椒第4代的目的基因进行了检测。发现所分离的柞蚕抗菌肽D基因与原设计的序列一样;辣椒组织中也含有抑菌物质,经与天然抗菌肽D对比,表明抗菌肽D基因得到了表达。
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| 关 键 词： | 转基因食品 柞蚕抗菌肽D基因 辣椒 表达产物 |
| 文章编号： | 0257-4799(2005)02-0155-06 |
| 修稿时间： | 2004-12-07 |
Detection of the Genetic Expression in the Cecropin Gene Transferred Capsicum |
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| LIAO Fupin,ZHONG Yangsheng,Deng Pingjian,Huang Ziran,LIU Jianjun,FANG Shisong,ZHAO Jin,CHEN Ying. Detection of the Genetic Expression in the Cecropin Gene Transferred Capsicum[J]. Acta Sericologica Sinica, 2005, 31(2): 155-160 |
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| Authors: | LIAO Fupin ZHONG Yangsheng Deng Pingjian Huang Ziran LIU Jianjun FANG Shisong ZHAO Jin CHEN Ying |
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| Abstract: | The nucleic acid and protein of the fourth generation of the cecropin gene transferred capsicum were identified by modern biotechnology methods. It is found that the separated cecropin D was the same as the original design sequence . Antibacterial protein was also found in the capsicum tissue . Constrasting this protein with the natural cecropin D showed that the cecropin D gene had been expressed in the capsicum .This experiment provides scientific basis for the safe detection of the transgenic food. |
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| Keywords: | Transgenic foods Antheraea pernyi cecropin D gene Capsicum Expression product |
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