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转柞蚕抗菌肽D基因辣椒的目的基因及表达产物检测
引用本文:廖富蘋,钟杨生,邓平建,黄自然,刘建军,房师松,赵锦,陈迎. 转柞蚕抗菌肽D基因辣椒的目的基因及表达产物检测[J]. 蚕业科学, 2005, 31(2): 155-160
作者姓名:廖富蘋  钟杨生  邓平建  黄自然  刘建军  房师松  赵锦  陈迎
作者单位:华南农业大学动物科学学院蚕丝科学系,广州,510642;深圳市疾病预防控制中心,深圳,518020
基金项目:国家自然科学基金 , 广东省科技厅科技计划
摘    要:应用现代生物技术,从核酸和蛋白质水平对转柞蚕抗菌肽D基因辣椒第4代的目的基因进行了检测。发现所分离的柞蚕抗菌肽D基因与原设计的序列一样;辣椒组织中也含有抑菌物质,经与天然抗菌肽D对比,表明抗菌肽D基因得到了表达。

关 键 词:转基因食品  柞蚕抗菌肽D基因  辣椒  表达产物
文章编号:0257-4799(2005)02-0155-06
修稿时间:2004-12-07

Detection of the Genetic Expression in the Cecropin Gene Transferred Capsicum
LIAO Fupin,ZHONG Yangsheng,Deng Pingjian,Huang Ziran,LIU Jianjun,FANG Shisong,ZHAO Jin,CHEN Ying. Detection of the Genetic Expression in the Cecropin Gene Transferred Capsicum[J]. Acta Sericologica Sinica, 2005, 31(2): 155-160
Authors:LIAO Fupin  ZHONG Yangsheng  Deng Pingjian  Huang Ziran  LIU Jianjun  FANG Shisong  ZHAO Jin  CHEN Ying
Abstract:The nucleic acid and protein of the fourth generation of the cecropin gene transferred capsicum were identified by modern biotechnology methods. It is found that the separated cecropin D was the same as the original design sequence . Antibacterial protein was also found in the capsicum tissue . Constrasting this protein with the natural cecropin D showed that the cecropin D gene had been expressed in the capsicum .This experiment provides scientific basis for the safe detection of the transgenic food.
Keywords:Transgenic foods  Antheraea pernyi cecropin D gene  Capsicum  Expression product
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