桑枝皮多糖的提取及组成成分与体外生物活性分析

为了更好地发掘桑枝皮的药用价值,采用热水浸提法从桑枝皮中分离提取多糖,其得率达到28.5%。采用薄层层析(TLC)法和非衍生化高效液相色谱(HPLC)法分析桑枝皮多糖的组成成分,初步推定桑枝皮多糖为一种杂多糖,由鼠李糖、木糖、阿拉伯糖、甘露糖、葡萄糖和半乳糖等单糖组成。桑枝皮多糖的体外生物活性试验显示其具有较强的对DPPH自由基的清除能力(IC50为1.7mg/mL)和对α-葡萄糖苷酶的抑制活性(IC50为0.298mg/mL)。上述结果提示桑枝皮多糖是降血糖和抗氧化的天然药物资源。

Extraction,Composition and in vitro Bioactivity of Polysaccharide from Mulberry Branch Bark

In order to facilitate exploitation of mulberry branch bark's medicinal value,hot water method was used to extract polysaccharide from mulberry branch bark and the extraction rate reached 28.5%.Composition of the polysaccharide was analyzed with thin-layer chromatography (TLC) and non-derivatization high performance liquid chromatography (HPLC).It was preliminarily inferred that the polysaccharide from mulberry branch bark was a mixed polysaccharide which contained rhamnose,xylose,arabinose,mannose,glucose and galactose etc.In vitro bioactivity assay indicated that mulberry branch bark polysaccharide had high scavenging ability to DPPH free radical (IC50=1.7 mg/mL) and strong inhibition activity to α-glucosidase (IC50=0.298 mg/mL).The above results suggest that mulberry branch bark polysaccharide is a natural medicinal resource with antidiabetes and antioxidant activities.