| 桑树多倍体育种材料遗传背景的AFLP分析 |
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| 引用本文： | 王卓伟,余茂德,鲁成,杨光伟,敬成俊.桑树多倍体育种材料遗传背景的AFLP分析[J].蚕业科学,2001,27(3):170-176. |
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| 作者姓名： | 王卓伟 余茂德 鲁成 杨光伟 敬成俊 |
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| 作者单位： | 农业部蚕桑学重点开放实验室西南农业大学, |
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| 基金项目： | 重庆市应用基础研究基金;; |
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| 摘 要： | 利用AFLP(Amplified Fragment Length Polymorphism)分子标记技术,即扩增片段长度多态性,从DNA分子水平对19份人工三倍体桑育种材料进行了遗传背景分析,构建了指纹图谱.19份育种材料可组配70个杂交组合,各材料间AFLP相似系数大于0.750 0的有54个组合,经AFLP分析认为,它们不宜作亲本间杂交组合;AFLP相似系数小于0.750 0的有16个组合,经AFLP分析认为它们适于作亲本间杂交组合.本研究从基因组DNA分子水平上为人工三倍体桑品种选育以及杂交亲本的选择和组配提供了遗传背景依据.
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| 关 键 词： | 桑树 多倍体 育种材料 遗传背景 AFLP分析 |
| 修稿时间： | 2000年12月11日 |
AFLP Analysis of Genetic Background of Polyploid Breeding Materials of Mulberry |
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| Wang Zhuowei,Yu Maode,Lu Cheng,Yang Guangwei,Jin Chengjun.AFLP Analysis of Genetic Background of Polyploid Breeding Materials of Mulberry[J].Acta Sericologica Sinica,2001,27(3):170-176. |
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| Authors: | Wang Zhuowei Yu Maode Lu Cheng Yang Guangwei Jin Chengjun |
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| Abstract: | The genetic background of 19 mulberry triploid breeding materials were analysed by means of AFLP(Amplified Fragment Length Polymorphism)molecular marker technique by which the fingerprint maps were obtained.70 hybridized combinations can be got from 19 breeding materials ,among which 54 hybridized combinations whose similarity coefficient is more than 0.750 0 are not suitable for breeding whereas 16 hybridized combinations whose similarity coefficient is less than 0.750 0 are suitable for breeding.The study provided the genetic background of materials for artificial triploid mulberry breeding on the level of genome DNA. |
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| Keywords: | Mulberry Polyploid Breeding materials Genetic background AFLP analysis |
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