siRNA 介导的家蚕ABC转运蛋白相关基因的干涉研究

由siRNA(smallinterferingRNA)介导的RNAi作为一种工具已被广泛用于基因功能研究中,并有望应用于生物体疾病的防治方面。通过显微注射,在家蚕中以siRNA介导的方式,对家蚕ABC转运蛋白(ATPbindingcassettetransporter)系列相关基因(Bmwh1,Bmwh2,Bmwh3)进行了siRNA干涉研究,在部分基因中得到了高效特异的干涉现象。结果初步证实正义链3′碱基错配的siRNA也具有显著的干涉效果。实验还显示了siRNA较之于dsRNA介导的RNAi更具有优势,表明siRNA介导的RNAi可作为基因功能分析和未来的基因操作手段在家蚕的研究中应用。

Study of siRNA-directed RNA Interference of the Genes Related ABC Transporter in Silkworm,Bombyx mori

RNA interference induced by small interfering RNA (siRNA) was widely used as a powerful tool to study gene function. It shows promising role in preventing and therapying diseases of organisms in the near future. Some RNAi phenotypes were achieved by injecting siRNA genes (Bmwh1,Bmwh2,Bmwh3) corresponding to the silkworm ATP Binding Cassette (ABC) Transporter. This was the first demonstration of siRNA-directed RNAi in silkworm. We confirmed that siRNA whose sense-stranded siRNA elements carry two nucleotide mismatches at the 3′-ends against the antisense-stranded siRNA elements, has an equal RNAi activity to the conventional siRNA. By comparison, siRNA has more special advantages than dsRNA in gene silencing. The results demonstrate that siRNA-induced RNAi has the potential that it can be applied on gene function exploration and gene manipulation in silkworm, Bombyx mori.