26种昆虫体内的1-脱氧野尻霉素含量测定

为发掘新的富含1-脱氧野尻霉素(DNJ)的昆虫资源,共测定了26种昆虫体内的DNJ含量。除家蚕(Bombyxmori)之外,野桑蚕(Bombyx mandarina)、桑蟥(Rondotia menciana)、桑尺蠖(Phthonandria atrineata)、桑螟(Diaphaniapyloalis)等4种仅取食桑叶的单食性或寡食性昆虫也富含DNJ,其中桑蟥和桑尺蠖幼虫中的DNJ质量分数分别为1.0648%±0.1134%、0.5138%±0.0083%,高于家蚕幼虫中的DNJ含量(0.4238%±0.0108%),并且桑尺蠖春季越冬代幼虫及其室内桑叶饲育的第1代幼虫和蛹、成虫均含有DNJ,具有开发利用价值。从桑园采集的兼性食桑昆虫中,人纹污灯蛾(Spilarectia subcarnea)、美国白蛾(Hlyphantria cunea)、大造桥虫(Ascotissele naria)、黄斑星天牛(Anoplophora nobilis)等4种昆虫的幼虫中含有微量DNJ,而褶翅尺蠖(Zamacra excauvata)、枣尺蠖(Sucra jujuba)、桑毛虫(Euproctis similis xanthocampa)幼虫和桑天牛(Apriona ger-mari)成虫中不含DNJ。豆天蛾幼虫(Clanis bilineata)等13种非食桑昆虫体内均不含DNJ。研究结果也提示取食桑叶的昆虫对DNJ的富集能力存在很大差异。

Determination of 1-Deoxynojimycin Contents in 26 Insect Species

In order to explore new insect resources rich in 1-deoxynojimycin (DNJ),DNJ contents in 26 insect species were determined. The results indicated that 4 mulberry monophagous or oligophagous insect species,namely Bombyx mandarina Leech,Rondotia menciana Moore,Phthonandria atrineata Butler,and Diaphaniapyloalis Walker,could also enrich DNJ besides the silkworm (Bombyx mori L. ). In Rondotia menciana Moore and Phthonandria atrineata Butler, DNJ contents were 1. 064 8% ±0. 113 4% and 0. 513 8% ± 0. 008 3% respectively,being higher than that in silkworm (0. 423 8% ±0. 010 8%). Moreover,the over-wintering larvae in spring,the first generation larvae,pupae and adults from indoor rearing of Phthonandria atrineata Butler all contained DNJ. These data indicated their value in further development. Among facultative insect species collected from mulberry field,larvae of Spilarectia subcarnea Walker,Hlyphantria cunea Drury,Ascotis selenaria Schiffermuller,and Anoplophora nobilis G. contained trace amount of DNJ,while Zamac-ra excauvata Dyer,Sucra jujuba Chu,Euproctis similis xanthocampa Dyar and Apriona germari Hope. did not contain DNJ. No DNJ was detected in 13 non-mulberry insect species including Clanis bilineata Walker. The obtained results also revealed that significant difference of the ability to enrich DNJ exists among the insect species feeding on mulberry.