桑萜类生物合成酶HMGR的生物信息学分析

萜类化合物是桑叶具有药用功效的主要化学成分之一。利用Vector NTI Suit 8等工具,对GenBank中的桑萜类生物合成关键酶3-羟基-3-甲基戊二酰辅酶A还原酶(HMGR)基因的核苷酸和氨基酸序列进行了生物信息学分析。桑hmgr基因序列以基因组DNA形式登录,其中1-5 905 bp区间为该基因序列,包括启动子、CDS和内含子序列,推测其编码蛋白的分子式为C2602H4181N715O786S29,亚细胞定位于线粒体,存在跨膜结构域,且含有22个氨基酸的质体转运肽,是含有HMG-CoA-reductase-classⅠ重要功能域的疏水性蛋白,二级结构以无规则卷曲和α-螺旋为主,三级结构中含有2个HMG-CoA和2个NADP(H)结合位点。

Bioinformatics Analysis of 3-hydroxy-3-methylglutaryl-CoA Reductase (HMGR) in Isoprenoid Biosynthesis of Mulberry

The nucleic acid sequences and amino acid sequences of the key isoprenoid synthase 3-hydroxy-3-methylglutaryl-CoA reductase(hmgr) gene from mulberry,which were registered in GenBank,were analyzed by some bioinformatics tools including Vector NTI Suit 8 and so on.The results showed that the nucleic acid sequences in GenBank is genomic DNA of hmgr,and its gene arranges from 1 to 5 905 bp,including promoter,coding sequence and intron.It can speculate that the HMGR protein lies in Mitochondria,with transmembrane topological structure and plastid transport peptide of 22 amino acid residues,and is a hydrophic protein with HMG-CoA-reductase-classⅠdomain.The secondary structure of HMGR mainly consists of α-helix and random coil and the tertiary structures contains two HMG-CoA binding motifs and two NADP(H) binding motifs.