| 柞蚕微孢子虫单克隆抗体的研制及诊断 |
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| 引用本文： | 唐啸尘,宁波.柞蚕微孢子虫单克隆抗体的研制及诊断[J].蚕业科学,1999,25(4):221-224. |
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| 作者姓名： | 唐啸尘 宁波 |
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| 作者单位： | 华南农业大学蚕桑系!广州510642(唐啸尘，黄自然)，中山医科大学微生物学教研室(宁波，张春艳) |
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| 摘 要： | 研究报告了柞蚕微孢子虫Nosemaantheraeae(简称N .a)单克隆抗体制备及免疫胶体金银染色法 (IGSS)诊断的结果。用抗N .a的单抗结合间接免疫胶体金银染色法 (IGSS)对 6种微孢子虫进行检测 ,在光学显微镜下柞蚕微孢子呈现特异的褐色 ,能与包括家蚕微孢子虫在内的其它 5种微孢子虫加以区别。
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| 关 键 词： | 柞蚕 微孢子虫 单克隆抗体 诊断 |
Detection of the Spores of Nosema antheraeae by Monoclonal Antibody Technique |
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| Tang Xiaochen,Huang Ziran.Detection of the Spores of Nosema antheraeae by Monoclonal Antibody Technique[J].Acta Sericologica Sinica,1999,25(4):221-224. |
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| Authors: | Tang Xiaochen Huang Ziran |
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| Abstract: | This study reported on preparation of monoclonal antibody of pebrine spore Nosema antheraeae of Chinese oak silkworm as well as on immunogold silver staining (IGSS) method for detecting pebrine spore of Chinese oak silkworm 6 kinds of pebrine spore are detected with the specific monoclonal antibody combining with IGSS method.The result shows that pebrine spore of Chinese oak silkworm is in specific brown color which is distinct from the other 5 kinds of pebrine spore including the spore of Nosema bombycis under optical microscope. |
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| Keywords: | Chinese oak silkworm Nosema antheraeae Monoclonal antibody Detection |
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