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基于piggyBac转座子转hGM-CSF基因家蚕的研究
引用本文:曹广力,薛仁宇,何泽,郑小坚,沈卫德,贡成良.基于piggyBac转座子转hGM-CSF基因家蚕的研究[J].蚕业科学,2006,32(3):324-328.
作者姓名:曹广力  薛仁宇  何泽  郑小坚  沈卫德  贡成良
作者单位:苏州大学生命科学学院,苏州,215123
基金项目:国家重点基础研究发展计划(973计划) , 国家自然科学基金
摘    要:将由家蚕核型多角体病毒IE-1基因启动子控制下的hGM-CSF基因克隆到p igA3GFP载体中,构建了家蚕转基因载体p igA3GFPIE-GMCSF],利用压力渗透法和精子介导法将其与辅助质粒helper p igA3一起导入家蚕蚕卵,获得产生绿色荧光的家蚕,次代产生荧光蚕的比例分别为0.17%,0.15%。将次代荧光蚕与正常蚕交配后代(G1)的荧光蚕个体再相互杂交,连续进行多代选育,获得了稳定遗传的转hGM-CSF基因家蚕品系。

关 键 词:piggyBac因子  转基因家蚕  人类粒细胞巨噬细胞集落刺激因子  IE-1启动子  精子介导法
文章编号:0257-4799(2006)03-0324-04
修稿时间:2006年3月6日

Research on hGM-CSF Transgentic Silkworm With piggyBac Transposon
CAO Guang-Li,XUE Ren-Yu,HE Ze,ZHENG Xiao-Jian,SHEN Wei-De,GONG Cheng-Liang.Research on hGM-CSF Transgentic Silkworm With piggyBac Transposon[J].Acta Sericologica Sinica,2006,32(3):324-328.
Authors:CAO Guang-Li  XUE Ren-Yu  HE Ze  ZHENG Xiao-Jian  SHEN Wei-De  GONG Cheng-Liang
Abstract:To approach hGM-CSF transgentic silkworm Bombyx mori,the victor pigA3GFP was constructed by cloning hGM-CSF with IE-1 promotor of BmNPV into pigA3GFP vector,and transported into eggs by pressure penetrating and sperm mediating together with the nonautonomous helper pigA3 which encodes piggyBac transposase.The ratio of green fluorescent larvae is 0.17% and 0.15% respectively in G_0 generation.G_1 was generated by mating G_0 green fluorescent silkworms with normal silkworms,then hybridization was carried out among the individuals of G_1 green fluorescent larvae.After continuously several hybridizations among green fluorescent silkworms,the steady transgenetic hGM-CSF silkworm strain was obtained.
Keywords:piggyBac transposon  Transgentic silkworm  hGM-CSF  IE-1 promotor  Sperm mediation
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