| 全蚕粉中1-脱氧野尻霉素的提取分离及分离物对肿瘤细胞的抑制作用 |
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| 引用本文： | 娄德帅,范涛,王储炎,颜辉,桂仲争.全蚕粉中1-脱氧野尻霉素的提取分离及分离物对肿瘤细胞的抑制作用[J].蚕业科学,2010,36(1):109-114. |
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| 作者姓名： | 娄德帅 范涛 王储炎 颜辉 桂仲争 |
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| 作者单位： | 江苏科技大学生物与环境工程学院,江苏镇江,212018;安徽省农业科学院蚕桑研究所,合肥,230061;江苏科技大学生物与环境工程学院,江苏镇江,212018;中国农业科学院蚕业研究所,江苏镇江,212018 |
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| 基金项目： | 公益性行业(农业)科研专项(No.nyhyzx07-020);;安徽省国际科技合作计划(No.09080703007) |
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| 摘 要： | 利用柱前衍生化反相高效液相色谱法检测不同前处理全蚕粉中1-脱氧野尻霉素(1-deoxynojirimycin,DNJ)的提取分离效果,并研究其分离产物对人宫颈癌HeLa细胞的抗肿瘤作用。结果表明,超低温冷冻干燥、热风烘干和真空浓缩煎煮冷冻干燥所得全蚕粉中DNJ的质量分数分别为0.494 9%、0.383 9%和0.607 6%;冷冻干燥全蚕粉粉碎粒度为0.15、0.25和0.425 mm时,DNJ质量分数分别为0.494 9%、0.321 5%和0.089 5%;用稀酸浸渍及阳离子交换树脂提取与分离全蚕粉中的DNJ效果最佳,分离物中DNJ的质量分数达到1.08%。浓度为40 mg/mL的全蚕粉分离物对肿瘤细胞的生长抑制率达92.27%,可使肿瘤细胞增殖能力完全丧失。结果提示:采用真空浓缩煎煮结合超低温冷冻干燥、超细粉碎和稀酸浸渍及阳离子交换树脂分离技术,可有效提高全蚕粉中DNJ的提取、分离效率,含DNJ的全蚕粉分离物对HeLa肿瘤细胞的生长具有显著的抑制作用。
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| 关 键 词： | 全蚕粉 1-脱氧野尻霉素 低温冻干 阳离子交换树脂分离 抗肿瘤 |
Isolation of 1-Deoxynojirimycin from Silkworm Powder and its Inhibitory Effect on Tumor Cells |
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| LOU De-Shuai,FAN Tao,WANG Chu-Yan,YAN Hui,GUI Zhong-Zheng.Isolation of 1-Deoxynojirimycin from Silkworm Powder and its Inhibitory Effect on Tumor Cells[J].Acta Sericologica Sinica,2010,36(1):109-114. |
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| Authors: | LOU De-Shuai FAN Tao WANG Chu-Yan YAN Hui GUI Zhong-Zheng |
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| Institution: | 1School of Biological and Environmental Engineering;Jiangsu University of Science and Technology;Zhenjiang Jiangsu 212018;China;2Sericultrual Research Institute;Anhui Academy of Agricultural Sciences;Hefei 230061;3The Sericultrual Research Institute;Chinese Academy of Agricultural Sciences;China |
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| Abstract: | By using the pre-column derivatization reverse phase high performance liquid chromatography,the efficiency of 1-deoxynojirimycin(DNJ) isolation from silkworm powder prepared with different pre-treatment methods was investigated.The obtained isolate was examined for their inhibitory effect to HeLa tumor cells.The results showed that,in the ultra-low temperature freeze-dried,heat-dried and vacuum condensation and decoction combining with ultra-low temperature freeze-dried,silkworm powder samples,DNJ content w... |
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| Keywords: | Silkworm powder Deoxynojirimycin (DNJ) Freeze drying Isolation with cation exchange resin Anti-tumor |
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