家蚕品种野三元耐微量拟除虫菊酯类农药污染新品系的筛选

基于为培育耐农药污染家蚕品种和研究家蚕对农药的耐受性机制提供基础材料的目的,对包括家蚕四元杂交品种野三元中2个含野桑蚕血统的中系亲本野A、野B和2个日系亲本84Y2、784等在内的160份家蚕品种资源,以0.01 mg/L杀灭菊酯添食后进行耐药性筛选,供试家蚕品种中的野B、辐射诱变品种辐7对微量杀灭菊酯具有较强的耐受能力。进一步以0.1～0.3 mg/L杀灭菊酯添食诱导后,自野A、野B中筛选建立了新的耐受性品系野AJ、野BJ,另以辐7为母本,分别以84Y2、784为父本杂交后培育获得新的耐受性品系辐84Y2、辐784。从以上4个新品系中均获得了添食微量杀灭菊酯后幼虫存活率100%的蛾区。4个对微量杀灭菊酯具有较强耐受性的新品系,进一步通过农药诱导选择及系统选择提高和固定其耐受性水平后,可作为耐农药污染新品种的育种材料。

Screening of New Lineages Tolerant To Pesticide Pollution of Trace Pyrethroid from Silkworm Variety Yesanyuan

In order to provide primary materials for cultivating silkworm(Bombyx mori) varieties tolerant to pesticide pollution and for studying the tolerance mechanism of silkworm to pesticides,tolerance to pesticide was investigated by administrating 0.01 mg/L fenvalerate to 160 silkworm variety resources including two Chinese strain parents Ye A and Ye B which have blood lineage of wild silkworm(Bombyx mandarina) and two Japanese strain parents 84Y2 and 784 of the four-way silkworm hybrid variety Yesanyuan.Among the tested silkworm varieties,Ye B and Fu 7,a radiation-induced mutant,had quite high tolerance to fenvalerate.Two new lineages,Ye AJ and Ye BJ,were established through direct selection from 0.1 ～ 0.3 mg/L fenvalerate treated individuals of Ye A and Ye B.Besides,Fu 7 was used as maternal parent,and 84Y2 and 784 as paternal parent respectively to breed two new lineages Fu 84Y2 and Fu 784 which had high torerance to fenvalerate.From the above four new lineages,we obtained moth batches in which the survival rate of silkworm larvae was 100% after treatment with trace fenvalerate.After being further improved and fixed the tolerance level,these four new lineages could be used as breeding materials of new silkworm varieties tolerant to pesticide pollution.