| 家蚕微孢子虫PCR检测的研究 |
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| 引用本文： | 蔡平钟,徐兴耀.家蚕微孢子虫PCR检测的研究[J].蚕业科学,1997,23(4):207-210. |
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| 作者姓名： | 蔡平钟 徐兴耀 |
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| 作者单位： | 四川省农业科学院(蔡平钟)，华南农业大学(徐兴耀，黄自然，卢铿明，庄楚雄)，中国热带农业科学院热带作物生物技术国家重点实验室(邵寒霜，刘志昕，郑学勤) |
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| 摘 要： | 根据家蚕微孢子虫（Nosemebombycis,N．b）孢子的DNA序列，设计合成一对引物，对家蚕微孢子虫孢子及其近缘种孢子的DNA进行PCR检测。结果表明只有家蚕微孢子虫孢子DNA获得特异性扩增区带，大小为317bp，可以区别于Nosemasp.MG1和MG2、柞蚕微孢子虫、Vairimorphanecatrix及Pleistophoraanguillarum等。对N.b孢子DNA检测灵敏度达1ng水平。
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| 关 键 词： | 家蚕微孢子虫 PCR检测 诊断 灵敏度 |
STUDIES ON PCR AMPLIFICATION OF DNA OF PEBRINE SPORE Nosema bombycis FROM SILKWORM,Bombyx mori |
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| Cai Pingzhong, Xu Xingyao, Huang Ziran ,Lu kengming ,Zhuang Chuxiong.STUDIES ON PCR AMPLIFICATION OF DNA OF PEBRINE SPORE Nosema bombycis FROM SILKWORM,Bombyx mori[J].Acta Sericologica Sinica,1997,23(4):207-210. |
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| Authors: | Cai Pingzhong Xu Xingyao Huang Ziran Lu kengming Zhuang Chuxiong |
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| Abstract: | Two primers were deslgned and synthesized according to the DNA sequence of spores of Nosema bomby(-is (Nb )from silkworm. The DNA of Nb spores and its related species (strain) were amplified separately by polymerase chain reaction (PCR ). The results showed a specific amplification in the DNA of Nb spore and the DNA fragment was about 317 bp, which distinguished N. bombycis from Nosema sp. MG1 and MG2, microsporidias of Antheraea pernyi, Vairimorpha necatrix, Pleitsophora anguillarum, etc.The detective sensitivity was. lng DNA. |
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| Keywords: | Nosema bombycis PCR Amplification Diagnosis Sensitivity |
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