酶法制备蚕茧层抗氧化多肽水解液的研究

基于利用蚕茧层开发抗氧化剂的目的,采用mu ltifect neutral细菌中性蛋白酶酶解蚕茧层,并用铁离子还原/抗氧化力分析法(FRAP)测定蚕茧层酶解液总抗氧化能力,确定原料预处理的温度和时间;运用正交试验优化酶解条件,比较蚕茧层多肽与丁基甲基苯酚(BHT)和VC的总抗氧化能力。结果表明:蚕茧层在100℃水浴加热2h后,添加质量分数为7.0%的mu ltifect neutral细菌中性蛋白酶,55℃下,pH 6.5、底物质量浓度0.033 3 g/mL、酶解210 m in时的水解物总抗氧化能力最强。

Preparation of Antioxidant Peptide Solution of Cocoon by Enzymatic Hydrolysis Method

On purpose of developing antioxidant from silkworm cocoon shells,bacterial neutral proteinase Multifect Neutral was used to hydrolyze cocoon,and the total antioxidation capacity(TAC) of hydrolysates was tested by FRAP method.The optimal hydrolysis parameters of neutral proteinase including temperature,time,pH,enzyme content and the substance concentration in enzymatic reaction system were determined by orthogonal design L_(16)(4)~(5),respectively.The results showed that the optimal conditions for producing protein hydrolysates with the highest TCA are 55 ℃,pH 6.5,enzyme content 7.0%,substance concentration(0.033 3 g/mL),and time duration 210 min.