促细胞生长再生复合丝素蛋白电纺丝非织布的性能

利用家蚕丝素蛋白独特的力学和生物学特性,以电纺蚕丝纤维为载体,通过加入含有细胞粘附构型肽段(TGRGDSPAS)8的纯化细胞粘连性蛋白,改善丝素蛋白在促细胞生长方面的生物学功能。红外光谱检测表明,加入10%的细胞粘连性蛋白并不会明显影响丝素蛋白电纺丝的α-螺旋和β-构象,细胞培养试验中也表现出促进细胞增殖的作用,其活性细胞数目较单纯丝素蛋白纤维上的活性细胞增加,约为1.5×104cm-2,并对细胞形态没有影响。电镜扫描显示,以加入10%细胞粘连性蛋白后的电纺蚕丝纤维直径仍呈均匀分布状态。依据研究结果认为,以加入细胞粘连性蛋白的再生复合丝素蛋白电纺丝非织布纤维作为生物医学材料,具有更加良好的应用前景。

Properties of the Electrospun Composite Fibroin Non-woven Fiber that Promotes Cell Proliferation

According to the unique mechanical and biological properties of silk fibroin, the electrospun silk fiber was used as the vector to improve the fibroin's biological functions on promoting cell proliferation through addition of purified cell-adhesive protein with the cell-adhesive peptide fragment (TGRGDSPAS)_8. The result of infrared spectrometry showed that 10% of cell-adhesive protein did not influence α helices and β conformations of the electrospun fibroin fiber obviously. Cell culture experiments indicated that addition of cell-adhesive protein promoted cell proliferation. The number of viable cells was higher than that on the single fibroin fiber while no adverse impact was observed on the cellular form. Electron microscopic scanning revealed that diameters of the electrospun silk fiber were still distributed evenly after the addition of 10% cell-adhesive protein. It is concluded that the electrospun composite fibroin non-woven fiber can have better application perspectives as biomedical materials after addition of cell-adhesive protein.