GST、6×His融合表达杆状病毒转移载体的构建

将pGEX-3X中由血吸虫（Schistosomajaponicum）编码的26kD谷胱甘肽转移酶基因Sj26和凝血因子Xa凝血酶位点引入到BacPAK8构建了融合表达杆状病毒转移载体BacSj26；将28kD的谷胱甘肽转移酶基因通过PCR突变消除终止密码后，引入BacPAK8构建了融合表达转移载体BacGST28。将P(MFHT)中的6×His序列克隆进BacPAK8，从而构建了6×His融合表达转移载体BacHis。这些融合表达转移载体的构建，为表达产物的一步纯化奠定了基础。

CONSTRUCTION OF GST、6×HIS FUSION EXPRESSION BACULOVIRUS TRANSFER VECTORS

The Schistosoma japonicum glutathione S-transferase gene Sj26 encoding 26kD protein and blood coagulation factor Xa sequence from the vector pGEX-3X were insertedinto vector BacPAK8 to generate Sj26 fusion expression baculovirus transfer vector Bacsj26. The glutathione S-transferase gene (GST) encoding 28kD protein was mutatedwith PCR amplication to exclude stop codon,then inserted into vector BacPAK8 to construct fusion expression transfer vector BacGST28. The nuclotide sequence encoding 6×Histidines from vector pMFHT was cloned into BacPAK8 to generate 6×His fusion expression transfer vector BacHis. All the fusion expression transfer vectors may lay a basis for the single-step purification of expression products.