桑叶多糖的提取与分析

以桑叶粉为材料,研究了不同提取条件对桑叶多糖提取效果的影响,并对桑叶多糖的组分进行了分析。桑叶多糖的最佳提取工艺为提取温度80℃、桑叶粉的质量浓度0100g/mL、提取次数3次、提取时间1h。提取物通过SephadexG-200柱层析检测得到3种多糖组分(Ⅰ、Ⅱ、Ⅲ),经SephadexG75柱层析测定3种多糖组分的相对分子量为41977、21220、6697。红外光谱表明多糖组分Ⅰ、Ⅲ含有呋喃糖苷,组分Ⅱ含有吡喃糖苷,组分Ⅰ、Ⅱ的结构中含有β糖苷键,组分Ⅲ的结构中含有α糖苷键。

The Extracting and Analyses of Polysaccharides in Mulberry Leaf

The effect of different extracting conditions on extracting effect and the ingredients of polysaccharides of mulberry leaf were studied.The optimal extracting conditions were:extracting temperature 80℃,material mass concentration 0.100 g/mL,extracting times 3 and extracting time 1 h.By SephadexG-200,the semi-finished products can be divided into three ingredients(Ⅰ,Ⅱ,Ⅲ).Every ingredient was collected and its relative molecular weight was 41 977,21 220,6 697 by SephadexG-75 separately.Infrared spectra analysis showed that the ingredientsⅠand Ⅲ contained glucosidefuranose,the ingredientⅡcontained glucosidepyranose.The structures of the ingredientsⅠandⅡhave β-glycobonds and the structure of ingredient Ⅲ contains α-glycobonds.