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A survey of porcine reproductive and respiratory syndrome among wild boar populations in Korea
Authors:Eun-Jin Choi  Chang-Hee Lee  Bang-Hun Hyun  Jae-Jo Kim  Seong-In Lim  Jae-Young Song  Yeun-Kyung Shin
Institution:Animal, Plant and Fisheries Quarantine and Inspection Agency, Anyang 430-855, Korea.
Abstract:No information is currently available on porcine reproductive and respiratory syndrome virus (PRRSV) infection in wild boars (Sus scrofa) in Korea. In this study, the status of PRRS in wild boars was investigated. Blood samples were collected from 267 wild boars from eight provinces in Korea. Four of the samples tested (1.5%) were positive for PRRSV antibodies and eight (3.0%) were positive for antigens. Of the virus-positive samples, three and five samples were typed as containing European (EU, type 1) or North American (NA, type 2) viruses, respectively. Two amplicons (one from type 1 and one from type 2) were used to analyze the PRRSV open reading frame 7 (ORF7) sequence. The nucleotide sequences of type 1 PRRSV ORF7 had identities between 96.1% and 98.4% with PRRSVs from domestic pigs in Korea. The sequences of type 2 PRRSV ORF7 had identities of 100% with the PRRSV strain VR-2332, which was prototypic North American strain. These results show that PRRSVs are present in wild boars in Korea, and effective PRRSV surveillance of the wild boar population might therefore be useful for disease control.
Keywords:ELISA  Korea  porcine reproductive and respiratory syndrome  RT-PCR  wild boar (Sus scrofa)
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