Plasmid profiling of Flavobacterium psychrophilum isolates from ayu (Plecoglossus altivelis altivelis) and other fish species in Japan |
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Authors: | Ji Hyung Kim Dennis K Gomez Toshihiro Nakai Se Chang Park |
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Institution: | 1Laboratory of Aquatic Animal Medicine & Brain Korea 21 Program for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 151-742, Korea.;2Fish Pathobiology Laboratory, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima 739-8528, Japan. |
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Abstract: | In order to evaluate the genetic variability of the causative agent of cold water disease (CWD), plasmid profiling was used to characterize Flavobacterium (F.) psychrophilum isolates (n = 169). Size analysis of plasmids in F. psychrophilum isolates (n = 128) from several fish species demonstrated that six kinds of plasmids were harbored, and ayu isolates had different profiles compared to other isolates. Moreover, multiple isolates (n = 41) from CWD outbreaks in 2002 to 2003 at a single ayu farm were examined to determine differences between isolates from successive outbreaks and showed different profiles by the sources of seedlings. |
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Keywords: | ayu CWD Flavobacterium psychrophilum plasmid profile |
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