| 副猪嗜血杆菌PCR快速诊断方法的建立 |
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| 引用本文： | 张盼锋,仇微,刘宇,张桂红,Zhang Guihong.副猪嗜血杆菌PCR快速诊断方法的建立[J].广东畜牧兽医科技,2009,34(1):33-36. |
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| 作者姓名： | 张盼锋 仇微 刘宇 张桂红 Zhang Guihong |
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| 作者单位： | 华南农业大学兽医学院,广东,广州,510642 |
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| 摘 要： | 副猪嗜血杆菌营养要求比较苛刻，常规生化试验检测方法比较烦琐，本研究针对副猪嗜血杆菌16SrRNA基因特异性PCR引物序列，合成一对PCR引物，建立相应的PCR检测方法，同时对该方法的灵敏性、特异性等实验。结果显示可检测出浓度为2．8×10^3CFU／mL的副猪嗜血杆菌表明该方法灵敏度高；对大肠杆菌、链球菌、巴氏杆菌、沙门氏杆菌、金黄色葡萄球菌进行PCR扩增均不获得任何条带，表明该方法特异性较强。所以该方法对于临床快速检测副猪嗜血杆菌具有重要意义。
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| 关 键 词： | 副猪嗜血杆菌 PCR 诊断 |
Establishment of a PCR Assay for Haemophilus Parasuis |
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| Zhang Panfeng,Qiu Weihong,Du Weiguo,Liu Yu,Zhang Guihong.Establishment of a PCR Assay for Haemophilus Parasuis[J].Guangdong Journal of Animal and Veterinary Science,2009,34(1):33-36. |
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| Authors: | Zhang Panfeng Qiu Weihong Du Weiguo Liu Yu Zhang Guihong |
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| Institution: | (College of Veterinary Medicine,South China Agricultural Uniwersity,Guangzhou 510642) |
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| Abstract: | Because of the fastidious growth and inconvenient traditional biochemical tests of Haemophitus parasuis, a PCR assay which amplified the gene of Haemophilus parasuis encoding 16S small subunit ribosomal RNA, was developed to detect Haemophilus parasuis,.The results showed that the sensitivity of this assay was 2.8 × 10^3 CFU/mL and could distinguish Haemophilus Parasuis with Avian Escherichia coli, Streptococcus, Pasteurella, Salmonella and Staphylococcus aureus. This sensitive and specific assay could be used to quickly detect Haemophilus parasuis in clinical samples. |
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| Keywords: | PCR |
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