| LUX^TM荧光PCR快速检测沙门氏菌的研究 |
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| 引用本文： | 许如苏,陈茹,林彩华,杨梓坚,陈冠武.LUX^TM荧光PCR快速检测沙门氏菌的研究[J].广东畜牧兽医科技,2009,34(6):30-34. |
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| 作者姓名： | 许如苏 陈茹 林彩华 杨梓坚 陈冠武 |
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| 作者单位： | 1. 汕头出入境检验检疫局,广东,汕头,515031
2. 广东出入境检验检疫局,广东,广州,510623 |
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| 基金项目： | 国家质检总局科研课题 |
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| 摘 要： | 本研究针对沙门氏菌invA基因的保守序列，设计特异的LUX^TM荧光标记引物，通过优化反应奈件和参数，建立可快速检测沙门氏菌的LUX^TM荧光PCR检测方法。结果显示，该方法高度敏感，其对纯菌的检测低限达到10^2cfu／mL，经6h增菌培养后检测，对样品液的检测低限达到1cfu／mL；特异性强，测试的全部13株沙门氏菌标准和参考菌株均呈阳性反应，测试的全部27株非目标菌均呈阴性反应；重复性好，定量检测批内和批间的变异系数均小于2％。应用本方法检测食品样品240份，结果检出阳性4份，与TaqMan荧光PCR和SN标准检测结果完全一致。本方法可在8h内完成对样品中沙门氏菌的检测，其检测的快速性、敏感性和特异性与TaqMan荧光PCR技术相当，且检测成本较低。
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| 关 键 词： | 沙门氏菌 LUXTM PCR TaqMan PCR 食品 |
Development of a LUX~(TM) PCR for rapid detection of Salmonella |
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| Xu Rusu,Chen Ru,Lin Caihua,Yang Zijian,Chen Guanwu.Development of a LUX~(TM) PCR for rapid detection of Salmonella[J].Guangdong Journal of Animal and Veterinary Science,2009,34(6):30-34. |
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| Authors: | Xu Rusu Chen Ru Lin Caihua Yang Zijian Chen Guanwu |
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| Institution: | Xu Rusu1,Chen Ru2,Lin Caihua1,Yang Zijian1,Chen Guanwu1 (1.Shantou Entry-Exit Inspection and Quarantine Bureau,Shantou 515031,China,2.Guangdong Entry-Exit Inspection and Quarantine Bureau,Guangzhou 510623,China) |
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| Abstract: | A LUXTM PCR method was developed for rapid detection of Salmonella based on the primers targeted for the conservative domain of invA gene of Salmonella using LUX self-quenched primer technology. The results showed that the method possessed high sensibility for detection of Salmonella and could detect as low as 102cfu/mL of Salmonella and as low as 1cfu/mL from 6h-cultured artificial contaminated samples. Thirteen standard and reference strains of Salmonella were all detected positive, and 27 reference/stand... |
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| Keywords: | LUXTMPCR TaqMan PCR |
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