| 小耳花猪蛔虫ITS及5.8S rDNA序列扩增与分析 |
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| 引用本文： | 吴晓斌,张志宏,谢东.小耳花猪蛔虫ITS及5.8S rDNA序列扩增与分析[J].广东畜牧兽医科技,2012,37(5):37-39. |
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| 作者姓名： | 吴晓斌 张志宏 谢东 |
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| 作者单位： | 江门市动物疫病预防控制中心,广东江门,529000 |
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| 摘 要： | 以分离自小耳花猪消化道内的蛔虫为研究对象,提取虫体的DNA片断,然后用引物对核糖体DNA(rDNA)的内转录间隔区ITS-1、ITS-2及5.8S序列进行PCR扩增。结果显示,目的片段ITS总长为1441 bp,2个不同样品之间的I TS序列没有差异。通过BLAST检索,与相关蛔虫I TS序列进行比较,发现与拜林蛔线虫(Bayl i sascari s t ransfuga)、猪蛔虫(Ascari s suum)和人蛔虫(Ascari s l umbri coi des)的ITS序列相似性分别为88%、98%和86%,与其他蛔虫的相似性均小于90%。
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| 关 键 词： | 蛔虫 PCR扩增 内转录间隔区(ITS) 序列分析 |
Amplification and Sequence Analysis of ITS and 5.8S rDNA of Ascarid Nematodes from Xiaocrhua Pig |
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| Wu Xiaobin,Zhang Zhihong,Xie Dong.Amplification and Sequence Analysis of ITS and 5.8S rDNA of Ascarid Nematodes from Xiaocrhua Pig[J].Guangdong Journal of Animal and Veterinary Science,2012,37(5):37-39. |
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| Authors: | Wu Xiaobin Zhang Zhihong Xie Dong |
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| Institution: | ( Jiangmen Animal Disease Prevention and Control Center, Jiangmen 529000, China ) |
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| Abstract: | Our research objective was to identify ascarid samples isolated from small ear floral pig. The ascarid DNA in small ear floral pig was extracted.The internal transcribed spacer （ITS） and 5.8S of ribosomal DNA （rDNA） of two ascarid samples were amplified by PCR using a pair of conserved primers.The lengths of ITS sequence were both 1441 bp long. The two ascarid specimens had identical ITS and 5.8S rDNA sequences.The sequences were blasted in GenBank and the similarities was 88%, 98%, 86% or below 90% with the sequences of Baylisascaris transfuga, Ascaris suum, Ascaris lumbricoides or other ascarid. |
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| Keywords: | Ascarids PCR amplification Internal transcribed spacers（ITS） Sequence analysis |
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