| 犬场蚊子与犬细小病毒 |
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| 引用本文： | 杨德威,宋延华.犬场蚊子与犬细小病毒[J].广东畜牧兽医科技,2000,25(4):16-18. |
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| 作者姓名： | 杨德威 宋延华 |
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| 作者单位： | 华南农业大学动物医学系,广东广州 |
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| 摘 要： | ２０００年４月，应用套式ＰＣＲ技术从犬场蚊子的血液样品检测出犬细小病毒。将分离到的犬细小病毒ＶＰ２－Ｙ３４基因片段克隆到ＰＭＤ１８－Ｔ载全，其病毒基因组ＣＰＶ－ＰＶ２－Ｙ３４序列测定结果显示与作者在ＧＥＮＥＢＡＮＫ发表的肺病变犬细小病毒ＣＰＶ－ＨＮ－１株有９９％同源性。
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| 关 键 词： | 犬细小病毒 蚊子 套式PCR 检测 |
Detection of Canine Parvovirus in Kennel Mosquitoes |
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| Waisees Yeung,SONG Yan-Hua,LIU Fu-An.Detection of Canine Parvovirus in Kennel Mosquitoes[J].Guangdong Journal of Animal and Veterinary Science,2000,25(4):16-18. |
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| Authors: | Waisees Yeung SONG Yan-Hua LIU Fu-An |
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| Abstract: | The presence of a vertically transmitted Canine parvovirus strain CPV/HN-1 in mosquitoes was revealed when screened with a Nested PCR in April 2000.Utilizing 2 stretches of nucleotide sequences repotred for the VP2 gene of canine parvovirus primers,DNA of the samples were subjected to a nested PCR procedure.The amplified Cloned VP2-Y3?Y4 fragment was cloned into a PMD18-T vector and the nucleotide sequence determined.Partial sequencing of the CPV-VP2-Y3?Y4 DNA fragment showed 99% nucleotide homology with that of the canine parvovirus,strain CPV/HN-1. |
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| Keywords: | Canine parvovirus Mosquito Nested PCR |
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