| 鸡SIgA的提取鉴定及其抗体制备 |
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| 引用本文： | 韩建保,王开功,于洪洋,周碧君,文明.鸡SIgA的提取鉴定及其抗体制备[J].贵州畜牧兽医,2008,32(3). |
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| 作者姓名： | 韩建保 王开功 于洪洋 周碧君 文明 |
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| 作者单位： | 贵州大学动物科学学院动物医学系,贵阳,550025 |
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| 摘 要： | 实验采用饱和硫酸铵法从健康肉鸡胆囊收集的胆汁中粗提二聚体SIgA,继而透析粗提产物除盐,然后进行纤维素DEAE-52离子交换层析,使用紫外分光光度计测定收集液的蛋白浓度,对层析产物浓缩使其浓度≥2.0 mg/m l,最后使用SDS-PAGE进行鉴定,从电泳所得图像中可以看到清晰的两个条带,一个条带分子量在67~70 ku之间为重链,另一个条带在26~28 ku之间为轻链。进一步将纯化的鸡SIgA抗体免疫健康兔,制备得到兔抗鸡SIgA抗体。
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| 关 键 词： | 鸡 二聚体 SigA 纤维素 DEAE-52 SDS-PAGE 兔抗鸡 SIgA |
Extraction, Identification and Antibody Preparation of Chicken SIgA |
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| Han Jianbao,Wang Kaigong,Yu Hongyang,Zhou Bijun,Wen Ming.Extraction, Identification and Antibody Preparation of Chicken SIgA[J].Guizhou Animal Science and Veterinary Medicine,2008,32(3). |
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| Authors: | Han Jianbao Wang Kaigong Yu Hongyang Zhou Bijun Wen Ming |
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| Abstract: | The experiment intends to abstract and identify the chicken secretory immunoglobulin A,and Preparation of Rabbit anti-chicken SIgA.Dimeride SIgA is originally abstracted from the chicken bile by the method of saturated ammonium sulfate,desalination by dialysis,cellulose DEAE-52 ion exchange chromatography,density the concentration of collected liquid by ultraviolet spectrophotometer,concentrate the laminar analysis production and make density greater than or equal to 2.0 mg/mL,identify the laminar analysis production by SDS-PAGE.We can find two limpid bands from the electrophoresis figure,one is the H-chain,which molecular weight is between 67KDa and 70KDa,the other is the L-chain,which molecular weight is between 26KDa and 28KDa.then,the healthy rabbit was immuned by SIgA,and gain the rabbit anti-chicken SIgA |
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| Keywords: | chicken Dimeride chicken secretory immunoglobulin A Cellulose DEAE-52 SDS-PAGE Rabbit anti-chicken SIgA |
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