白蚁肠道可培养菌群及其功能初探

白蚁是一种危害严重的昆虫,其以破坏木质而家喻户晓.文献表明,白蚁对木质强大的消化能力很大程度上取决于其肠道微生物的影响.为了探究白蚁肠道微生物的类群,本试验以黑翅土白蚁为材料,分离纯化获得其肠道主要可培养微生物的纯培养112种,其中喜氧菌35种,厌氧菌77种;在分离出的微生物类群中,通过刚果红法初步筛选出12种喜氧菌菌株可能具有纤维素酶活性,其最大透明圈达到4.0㎝×4.0㎝;进一步检测这些菌株的纤维素酶活性发现,12株菌均具有羧甲基纤维素钠盐(CMC-NA)酶活性和滤纸酶活性,但是大部分菌株羧甲基纤维素酶活性较弱,而一些菌株如 NBY14滤纸酶活较高,可达到16.188u/mL.试验结果可为黑翅土白蚁防治提供依据,亦可为纤维素酶的开发利用奠定基础.

The Microbes Cultured and Their Function in the Gut of Termites

Termites,an important pest,were known by everyone because of their damage to the wood.It is suggested that the digestive ability of termites on wood depends mainly on the intestinal microbes in some literatures.In order to know the microbes in the gut of termites,in this experiment,by regarding Odontotermes formosanus as materials,112 species were separated and purified from the gut of termites,and 35 strains are aerophile and 77 strains are anaerobic.In all microorganisms,12 aerophile species,which may have the activity of cellulose,were screened by Congo red method initially.the largest transparent circle get up to 4.0 ㎝×4.0 ㎝.Further,the cellulase activities of 12 strains were checked.The results showed that the sodium carboxy methyl cellulose(CMC-Na) enzyme activity and the filter paper enzyme activity were presented in the ferment liquid of 12 strains,but the sodium carboxy methyl cellulose enzyme activity of numerous strains is lower and the filter paper enzyme activity of some strains,eg.strain NBY14 is higher up to 16.188 u/ml.It is significant that the results not only provide the basis for controlling the termite but also lay the foundation for the development and utilization of cellulose.