| 甘薯脱毒技术及增产效果研究 |
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| 引用本文： | 杨崇良,尚佑芬,赵玖华,李长松.甘薯脱毒技术及增产效果研究[J].植物保护学报,1998,25(1):52-55. |
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| 作者姓名： | 杨崇良 尚佑芬 赵玖华 李长松 |
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| 作者单位： | 山东省农业科学院植物保护研究所，山东省农业科学院植物保护研究所，山东省农业科学院植物保护研究所，山东省农业科学院植物保护研究所 济南 250100，济南 250100，济南 250100，济南 250100 |
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| 基金项目： | 山东省计划委员会“八五”攻关 |
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| 摘 要： | 作者于１９８８～１９９５年研究了甘薯病毒及甘薯脱毒技术，明确了侵染山东甘薯的主要病毒种类是甘薯羽状斑驳病毒和甘薯潜隐病毒，在国内首次分离侵染甘薯的烟草花叶病毒，探明了其生物学特性。筛选出适合山东甘薯茎尖培养基的最佳激素配比，浓度，ｐＨ值。探索了脱毒薯的增产机理和增产效果，提出了组织培养，茎尖苗检测，脱毒薯速率与推广应用的配套技术规程，培养出徐薯１８等１２个品种的脱毒苗，平均增产４２．９％出干率提高
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| 关 键 词： | 甘薯 病毒病 毒原鉴定 脱毒 病毒检测 茎尖苗 |
PRODUCE TECHNIQUES AND PRACTICE OF VIRUS-FREE SWEET POTATO |
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| Yang Chongliang,Shang Youfen,Zhao Jiuhua and Li Changsong.PRODUCE TECHNIQUES AND PRACTICE OF VIRUS-FREE SWEET POTATO[J].Acta Phytophylacica Sinica,1998,25(1):52-55. |
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| Authors: | Yang Chongliang Shang Youfen Zhao Jiuhua and Li Changsong |
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| Institution: | Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan,Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan,Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan and Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan |
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| Abstract: | The sweet potato viruses and the techniques to get virus-free sweet potato was studied in 1988 - 1995. It was clarified that the main sweet potato viruses were SPFMV and SPLV in Shandong Province. TMV infecting sweet potato was isolated for the first time in China. The biological features were studied. The medium for culturing virus-free sweet potato was screened. The mechanism of yield increase of virus-free sweet potato and the effect of yield increase were investigated. A complete technique process for application of virus-free sweet potato including apical meristem culture, virus test of regenerated plants, virus-free sweet potato propagation was proposed. Twelve cultivars including Xushu 18 were cultivated. The yield of virus-free sweet potato increased 42. 9% in average compared to the control, which was infected by the virus. The infection of Monilochaetes infuscans, Ceratocystis fimbreata and Prathlenchus coffeac decreased 90% in comparison with the control. |
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| Keywords: | sweet potato virus virus identification virus-free technique virus test of apical meristem |
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