| 进境油菜籽中黑胫病菌和茎基溃疡病菌的检测 |
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| 引用本文： | 周国梁,尚琳琳,于翠,印丽萍,徐殿胜,易建平.进境油菜籽中黑胫病菌和茎基溃疡病菌的检测[J].植物保护学报,2010,37(4):289-294. |
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| 作者姓名： | 周国梁 尚琳琳 于翠 印丽萍 徐殿胜 易建平 |
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| 作者单位： | 1. 上海出入境检验检疫局,上海,200135
2. 华东理工大学生物工程学院,上海,200237 |
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| 基金项目： | 上海出入境检验检疫局科研项目(HK069-2010);上海市科委项目(09DZ0580201) |
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| 摘 要： | 为准确鉴定从进境澳大利亚油菜籽样品中分离的真菌分离物,利用形态学特征、PCR检测、序列分析以及致病性测试等方法对分离物6382-43和6382-51进行了鉴定试验。结果表明,分离物6382-43的形态特征和油菜茎基溃疡病菌Leptosphaeria maculans相似,菌丝生长较慢,菌落边缘不规则,不产生色素。油菜茎基溃疡病菌特异性引物LmacF/LmacR检测为PCR阳性;ITS区序列和油菜茎基溃疡病菌的序列相似性为99.8%;接种幼嫩油菜子叶产生油菜茎基溃疡病的典型症状。分离物6382-51的形态特征和油菜黑胫病菌L.biglobosa相似,菌丝生长较快,菌落边缘规则,产生色素;油菜黑胫病菌特异性引物LbigF/LmacR检测为PCR阳性;ITS区序列和油菜黑胫病菌的序列相似性为100%;接种幼嫩油菜子叶产生油菜黑胫病的典型症状。根据分离物的形态特征、PCR检测结果、序列分析以及致病性测试结果,将进境澳大利亚油菜籽样品中的真菌分离物6382-43和6382-51分别鉴定为油菜茎基溃疡病菌Leptosphaeria maculans和油菜黑胫病菌L.biglobosa。
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| 关 键 词： | 油菜籽 油菜茎基溃疡病菌 油菜黑胫病菌 检测 |
| 收稿时间： | 2010/1/11 0:00:00 |
Detection of Leptosphaeria maculans and L.biglobosain oilseed rape samples imported from Australia |
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| Zhou Guoliang,Shang Linlin,Yu Cui,Yin Liping,Xu Diansheng and Yi Jianping.Detection of Leptosphaeria maculans and L.biglobosain oilseed rape samples imported from Australia[J].Acta Phytophylacica Sinica,2010,37(4):289-294. |
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| Authors: | Zhou Guoliang Shang Linlin Yu Cui Yin Liping Xu Diansheng and Yi Jianping |
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| Institution: | Shanghai Export and Import Inspection and Quarantine Bureau, Shanghai 200135, China;East China University of Science and Technology, Shanghai 200237, China;Shanghai Export and Import Inspection and Quarantine Bureau, Shanghai 200135, China;Shanghai Export and Import Inspection and Quarantine Bureau, Shanghai 200135, China;East China University of Science and Technology, Shanghai 200237, China;Shanghai Export and Import Inspection and Quarantine Bureau, Shanghai 200135, China |
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| Abstract: | The isolates 6382-43 and 6382-51 were intercepted from oilseed rape samples imported from Australia.The isolate 6382-43 with similar morphological characteristics to Leptosphaeria maculans showed a slower growth rate of 3.0 mm/d with irregular margin of colony and no pigment production.The isolate 6382-51 with a faster growth rate of 4.8 mm/d and regular margin of colony produced diffusible pigment.The result of PCR detection showed that the isolate 6382-43 could be amplified by L.maculans-specific primer L... |
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| Keywords: | Brassica napus seed Leptosphaeria maculans Leptosphaeria biglobosa detection |
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