| 油茶根腐病拮抗内生细菌的筛选及鉴定 |
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| 引用本文： | 宋光桃,周国英,刘君昂,李河,李琳.油茶根腐病拮抗内生细菌的筛选及鉴定[J].植物保护学报,2010,37(2):137-142. |
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| 作者姓名： | 宋光桃 周国英 刘君昂 李河 李琳 |
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| 作者单位： | 1. 中南林业科技大学,长沙,410004;湖南环境生物职业技术学院,衡阳,421005
2. 中南林业科技大学,长沙,410004 |
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| 基金项目： | 国家"十一五"科技支撑计划(2009BADB1B0204;2006BAD08A1104);湖南研究生科研创新基金(2009b165);中南林业科技 大学研究生科技创新基金(2007bx02) |
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| 摘 要： | 为探讨防治油茶根腐病的高效拮抗菌的拮抗机制,采用研磨法从健康油茶根、茎、叶、果等组织中分离内生菌,通过平板对峙试验和抑菌谱检测试验筛选对油茶根腐病菌具有高效拮抗作用的内生菌,并进行形态观察、生理生化指标测定和16S rRNA序列分析.结果显示:从油茶中分离获得内生细菌75株,对油茶根腐病菌有拮抗作用的菌株18株,其中抑菌圈半径在5 mm以上的6株.这6株细菌的发酵液对油茶根腐病菌等6种供试病原真菌均有拮抗作用,其中菌株R6的抑菌效果最好,对各病原菌的抑菌圈半径均在10 mm以上.根据对R6的形态观察和生理生化指标测定,以及16S rRNA序列分析结果,初步鉴定其为枯草芽孢杆菌Bacillus subtilis.
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| 关 键 词： | 拮抗 内生细菌 鉴定 油茶根腐病 |
| 收稿时间： | 2009/8/14 0:00:00 |
Isolation and characterization of antagonistic endophytic bacteria again Fusarium proliferatum |
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| Song Guangtao,Zhou Guoying,Liu Junli,Li He and Li Lin.Isolation and characterization of antagonistic endophytic bacteria again Fusarium proliferatum[J].Acta Phytophylacica Sinica,2010,37(2):137-142. |
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| Authors: | Song Guangtao Zhou Guoying Liu Junli Li He and Li Lin |
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| Institution: | Central South University of Forestry Sciences & Technology, Changsha 410004, Hunan Province, China; Hunan Environment-Biologic Polytechnic, Hengyang 421005, Hunan Province, China;Central South University of Forestry Sciences & Technology, Changsha 410004, Hunan Province, China;Central South University of Forestry Sciences & Technology, Changsha 410004, Hunan Province, China;Central South University of Forestry Sciences & Technology, Changsha 410004, Hunan Province, China;Central South University of Forestry Sciences & Technology, Changsha 410004, Hunan Province, China |
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| Abstract: | For discussing the antagonistic mechanism of the high efficient endophytic bacteria to control the root rot disease of Camellia oleifera caused by Fusarium proliferatum, we isolated the endophytic bacteria from healthy roots, stems, leaves and fruits of C.oleifera plants, screened the high efficient endophytic bacteria against Fusarium proliferatum by the plate confrontation tests and inhibition spectrum tests, and then identified the strain according to the characteristics of morphology, physiology and bio... |
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| Keywords: | antagonism endophytic bacteria identification Fusarium proliferatum |
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