| 甘蔗宿根矮化病菌巢式PCR检测 |
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| 引用本文： | 沈万宽,刘睿,邓海华.甘蔗宿根矮化病菌巢式PCR检测[J].植物保护学报,2012,39(6):508-512. |
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| 作者姓名： | 沈万宽 刘睿 邓海华 |
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| 作者单位： | 广州甘蔗糖业研究所,广东省甘蔗改良与生物炼制重点实验室,广州510316 |
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| 基金项目： | 广东省科技攻关项目(2006A20207001),科技部科研院所开发专项资金项目(2008EG11203),广东省科技计划(粤科函财字[2009]627号),国家甘蔗产业技术体系建设专项资金项目(CARS-20) |
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| 摘 要： | 为了探索甘蔗宿根矮化病快速检测技术,以细菌16S-23S rDNA内转录间隔区(ITS)通用引物ITS1/ITS2为第1轮引物,以甘蔗宿根矮化病菌特异引物Lxx1/Lxx2为第2轮引物,建立甘蔗宿根矮化病菌巢式PCR检测技术。巢式PCR能扩增出438 bp的目的条带,其核苷酸序列与巴西、澳大利亚及美国甘蔗宿根矮化病菌分离物核苷酸序列同源率为100%或99.8%;其检测灵敏度为10fg甘蔗宿根矮化病菌基因组DNA,较常规PCR提高100倍;样品检测结果也表明巢式PCR检测灵敏度明显优于常规PCR,可用于+1片嫩叶和心叶等微量病菌样品的检测。
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| 关 键 词： | 甘蔗 宿根矮化病菌 巢式PCR |
| 收稿时间： | 3/3/2012 12:00:00 AM |
Nested-PCR detection for Leifsonia xyli xyli, the causal bacterium of sugarcane ratoon stunting disease |
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| Shen Wankuan,Liu Rui and Deng Haihua.Nested-PCR detection for Leifsonia xyli xyli, the causal bacterium of sugarcane ratoon stunting disease[J].Acta Phytophylacica Sinica,2012,39(6):508-512. |
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| Authors: | Shen Wankuan Liu Rui and Deng Haihua |
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| Institution: | Guangzhou Sugarcane Industry Research Institute/Guangdong Key Laboratory of Sugarcane Improvement and Biorefinery, Guangzhou 510316, Guangdong Province, China;Guangzhou Sugarcane Industry Research Institute/Guangdong Key Laboratory of Sugarcane Improvement and Biorefinery, Guangzhou 510316, Guangdong Province, China;Guangzhou Sugarcane Industry Research Institute/Guangdong Key Laboratory of Sugarcane Improvement and Biorefinery, Guangzhou 510316, Guangdong Province, China |
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| Abstract: | |
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| Keywords: | sugarcane Leifsonia xyli xyli nested-PCR |
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