山东省冬小麦田猪殃殃抗药性水平及ASL靶标抗性机理分析

为明确山东省冬小麦田猪殃殃Galium aparine对常规除草剂氯氟吡氧乙酸、苯磺隆及双氟磺草胺的抗性水平和抗性种群的乙酰乳酸合成酶(acetolactate synthase,ALS)靶标抗性机理,在温室内采用整株生物测定法测定21个猪殃殃种群对氯氟吡氧乙酸、苯磺隆和双氟磺草胺的抗性水平,同时根据猪殃殃ALS基因序列设计引物,提取猪殃殃高抗种群单株基因组DNA进行测序,并与拟南芥Arabidopsis thaliana敏感型ALS基因进行比对,查找突变位点分析其抗性机理。结果表明,21个猪殃殃种群对氯氟吡氧乙酸均敏感,尚未产生抗性;90.48%的猪殃殃种群已对苯磺隆产生了抗性,其中低抗、中抗和高抗种群分别占总种群数的23.81%、23.81%和42.86%,相对抗性指数最高为1 134.82;71.43%的猪殃殃种群已对双氟磺草胺产生了抗性,其中低抗、中抗和高抗种群分别占总种群数的19.05%、9.52%和38.10%,相对抗性指数最高为87.05。高抗苯磺隆种群XZ-1和LW均发生了ALS基因第197位氨基酸功能位点的突变,其中XZ-1种群发生了CCC(脯氨酸)到TCC(丝氨酸)...

Determination of the resistance level of cleavers Galium aparine in winter wheat fields in Shandong Province to common herbicides and its resistance mechanism to the ALS target

In order to determine the resistance levels of cleavers Galium aparine to fluroxypyr, florasulam and tribenuron-methly and its resistance mechanism to the acetolactate synthase (ALS) target, the whole-plant dose response experiments were conducted to determine the resistance levels of 21 G. aparine populations collected in Shandong Province to the three herbicides in glasshouses. At the same time, the genomic DNA of a single plant with high resistance to tested herbicides was extracted, and the ALS gene was sequenced according to the reported gene sequence of G. aparine. The obtained sequence was then compared with that of the susceptible type of Arabidopsis thaliana to find out the mutation site and the resistance mechanism. The results showed that all populations of G. aparine were sensitive to fluroxypyr, but 90.48% of them were resistant to tribenuron methyl, including 23.81% slightly resistant, 23.81% moderately resistant and 42.86% highly resistant populations, and the highest relative resistance was 1 134.82. In contrast, 71.43% populations were resistant to florasulam, including 19.05% slightly resistant, 9.52% moderately resistant and 38.10% highly resistant populations, and the highest relative resistance was 87.05. Sequence analysis showed that there was a point mutation from CCC (Pro) to TCC (Ser) and from CCC (Pro) to ACC (Thr) at the position 197 of ALS gene in highly resistant populations XZ-1 and LW, respectively, suggesting that the position 197 might be the main point mutation site of G. aparine resistant to tribenuron-methly and florasulam.