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| 灰葡萄孢菌激活蛋白PEBC2的生物功能 | |
| 引用本文： | 张云华,邱德文,张立军,杨秀芬,曾洪梅,袁京京.灰葡萄孢菌激活蛋白PEBC2的生物功能[J].植物保护学报,2008,35(2):123-126. |
| 作者姓名： | 张云华 邱德文 张立军 杨秀芬 曾洪梅 袁京京 |
| 作者单位： | 1. 沈阳农业大学,沈阳,110161;中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京,100081 2. 中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京,100081 3. 沈阳农业大学,沈阳,110161 |
| 基金项目： | 国家高技术研究发展计划(863计划) , 北京市科技计划 |
| 摘 要： | 通过沸水浴、离心、阴离子交换层析等方法,从灰葡萄孢菌Botrytis cinerea BC-4-2-2-1菌丝体中分离纯化出一种激活蛋白,命名为PEBC2,经SDS-PAGE银染显示呈单一条带,相对分子量约为14kD。该激活蛋白能显著提高小麦种子的发芽率,促进小麦幼苗生长,增强小麦的抗旱性和番茄对灰霉病的抗性。经激活蛋白处理后小麦发芽率从75.00%提高到98.33%,处理7、9、11天时株高分别比对照增加了17.07%、23.05% 和17.19%,幼苗抗旱综合系数从36.53提高到57.45;该激活蛋白对番茄灰霉病的诱抗效果达60.09%。 |
| 关 键 词： | 灰葡萄孢菌 激活蛋白PEBC2 纯化 生物活性 灰葡萄孢菌 激活蛋白 生物功能 Botrytis cinerea protein activator bioactivity 效果 番茄灰霉病 系数 综合 抗旱性 幼苗生长 株高 处理 抗性 增强 小麦种子 发芽率 相对分子量 |
| 收稿时间： | 2007/12/12 0:00:00 |
| 修稿时间： | 2007/12/12 0:00:00 |
Purification and its bioactivity of an activator protein PEBC2 from Botrytis cinerea |
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| Zhang Yunhu,Qiu Dewen,Zhang Lijun,Yang Xiufen,Zeng Hongmei and Yuan Jingjing.Purification and its bioactivity of an activator protein PEBC2 from Botrytis cinerea[J].Acta Phytophylacica Sinica,2008,35(2):123-126. | |
| Authors: | Zhang Yunhu Qiu Dewen Zhang Lijun Yang Xiufen Zeng Hongmei and Yuan Jingjing |
| Institution: | Shenyang Agricultural University, Shenyang 110161, Liaoning Province, China;The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China;The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China;Shenyang Agricultural University, Shenyang 110161, Liaoning Province, China;The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China;The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China;The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
| Abstract: | |
| Keywords: | Botrytis cinerea activator protein PEBC2 purification bioactivity |
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