放线菌11-3-1对油菜菌核病的防治作用与菌株鉴定

为了进一步明确放线菌11-3-1菌株的生防效果和分类地位,采用平板对峙法和活体组织法测定其抑菌效果。放线菌11-3-1菌株对供试的15种植物病原真菌菌丝生长均有不同程度的抑制作用,尤其对油菜菌核病菌具有明显抑制作用,抑菌带可达到19.67 mm。显微镜观察显示,11-3-1菌株对油菜菌核病菌菌丝生长有明显影响,可致使油菜菌核病菌菌丝体畸形、扭曲和原生质浓缩外渗等。11-3-1菌株发酵原液对油菜菌核病的室内药效达96.91%,效果显著优于对照组和药剂对照组。根据形态观察、生理生化特性和16S rDNA序列比对分析,初步鉴定该菌株为黄色长孢链霉菌Streptomyces longisporoflavus。

Antifungal activity against rapeseed Sclerotinia stem rot and identification of actinomycete strain 11-3-1

In order to ensure the efficacy and its taxonomic status of actinomycete strain 11-3-1, agar block method and tissue culture method were used to test the antifungal activity. The results showed that the strain displayed a broad-spectrum inhibitory activity against 15 plant pathogenic fungi, especially to Sclerotinia sclerotiorum, the width of inhibition zones was up to 19.67 mm. Strain 11-3-1 induced alterations on hyphae morphology of S.sclerotiorum, such as distortion, cytoplasm exosmosis and deformation. Tissue culture method tests showed that the inhibitions rates of the fermentation broth was 96.91% to S.sclerotiorum, and fermentation broth had a better control effect than CK group and fungicide group. According to the morphological, physiological and biochemical characteristics, and 16S rDNA sequence, the strain 11-3-1 was identified as Streptomyces longisporoflavus.