西花蓟马抗甲氨基阿维菌素苯甲酸盐种群的交互抗性与生化抗性机制

为了解西花蓟马Frankliniella occidentalis(Pergande)对甲氨基阿维菌素苯甲酸盐的抗性风险,采用生物和生化测定方法研究了西花蓟马甲维盐抗性种群与其它杀虫剂的交互抗性和生化抗性机制。西花蓟马甲维盐抗性种群对阿维菌素有高水平交互抗性,抗性倍数为31.656,对啶虫脒有中等水平交互抗性,为12.182,对吡虫啉、溴虫腈、氯氟氰菊酯、毒死蜱和灭多威有低水平交互抗性,为5.517~8.568,而对多杀菌素无明显交互抗性。增效剂胡椒基丁醚(PBO)、马来酸二乙酯(DEM)、三丁基三硫磷酸酯(DEF)和磷酸三苯酯(TPP)对甲维盐抗性种群和田间种群均有显著增效作用。甲维盐抗性种群多功能氧化酶细胞色素P₄₅₀和b₅含量、O-脱甲基酶、谷胱甘肽S-转移酶和羧酸酯酶活性均显著提高,分别为敏感种群的3.89、3.61、5.32、4.42和1.30倍,表明多功能氧化酶、谷胱甘肽S-转移酶和羧酸酯酶等解毒代谢酶活性的提高是西花蓟马对甲维盐产生抗性的重要机制。

Cross-resistance and biochemical resistance mechanisms of emamectin-benzoate resistant population of Frankliniella occidentalis

In order to evaluate the resistance risk of emamectin-benzoate to Frankliniella occidentalis (Pergande), the cross-resistance and biochemical resistance mechanisms of emamectin benzoate resistant population were investigated using the methods of Munger cell, synergist and enzyme activity assay. The results showed that the resistance population had high level of cross-resistance to abamectin with 31.656-fold, medium level of cross-resistance to acetamiprid with 12.182-fold, low level of cross-resistance to imidacloprid, chlorfenapyr, cyhalothrin, chlorpyrifos and methomyl with 5.517-8.568-fold, yet no cross-resistance to spinosad. The synergists piperonyl butoxide (PBO), diethyl maleate (DEM), s, s, s-tributyl phosphorotrithioate (DEF) and triphenyl phosphate (TPP) all had significant synergism to the emamectin-benzoate resistance- and field populations. Compared with the susceptible population, the emamectin-benzoate resistance population had significantly increased contents of cytochrome P₄₅₀ and b₅, activities of mixed-functional oxidases O-demethylase, glutathione S-transferases and carboxylesterase with 3.89, 3.61, 5.32, 4.42 and 1.30 fold increase, respectively. Therefore, it was inferred that the increased detoxification enzyme activities may play an important role in the resistance of F.occidentalis to emamectin-benzoate.