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| 转抗真菌基因水稻对纹枯病的抗性表达分析 | |
| 引用本文： | 袁红旭,许新萍,张燕娟,刘晚苟,郭建夫. 转抗真菌基因水稻对纹枯病的抗性表达分析[J]. 植物保护学报, 2009, 36(6): 504-508 |
| 作者姓名： | 袁红旭 许新萍 张燕娟 刘晚苟 郭建夫 |
| 作者单位： | 1. 湛江师范学院生物科学与技术学院,湛江,524048;广东海洋大学农业生物技术中心,湛江,524088 2. 中山大学生物工程中心,广州,510275 3. 广东海洋大学农业生物技术中心,湛江,524088 4. 湛江师范学院生物科学与技术学院,湛江,524048 |
| 基金项目： | 国家"863"高新技术发展计划(10101-02-02),转基因生物新品种培育科技重大专项(2009ZX08011-02913),湛江师范学院教 授基金(2009-1) 作者简介: 袁红旭,女,1964年生,教授,研究方向为植物免疫学与微生物学, email: yuanhx588@sohu.com |
| 摘 要： | 采用人工接种和酶活性测定的方法,研究转入几丁质酶基因等1～4个抗真菌基因的13个转基因水稻株系对纹枯病的抗性和几丁质酶活性变化规律.结果表明:13个转基因水稻株系的抗性均显著高于非转基因亲本.母本抗性水平不同或者导入单个及多个抗真菌基因的转基因水稻中均可筛选出抗性优良的株系;转基因水稻病情指数的变异系数为21.93～69.32,高于非转基因亲本的14.37～47.00,表明转基因水稻的抗病性更容易变异.对转基因水稻几丁质酶的活性分析结果表明,除1个转基因水稻株系的酶活性与非转基因亲本相同外,其它转基因水稻酶活性均高于非转基因亲本,但酶活力高低与转基因水稻株系间抗病性的高低无显著的相关性. |
| 关 键 词： | 转基因水稻 纹枯病 抗病性 几丁质酶 |
| 收稿时间： | 2009-01-28 |
Analysis on resistance to sheath blight of transgenic rice with extra anti-fungual genes |
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| Yuan Hongxu,Xu Xinping,Zhang Yanjuan,Liu Wangou and Guo Jianfu. Analysis on resistance to sheath blight of transgenic rice with extra anti-fungual genes[J]. Acta Phytophylacica Sinica, 2009, 36(6): 504-508 | |
| Authors: | Yuan Hongxu Xu Xinping Zhang Yanjuan Liu Wangou Guo Jianfu |
| Affiliation: | Life Science and Technology School of Zhanjiang Normal University, Zhanjiang 524048, Guangdong Province, China;Agricultural Institute of Guangdong Ocean University, Zhanjiang 524088, Guangdong Province, China;Biotechnology Research Center of Sun Yat-sen University, Guangzhou 510275, Guangdong Province, China;Agricultural Institute of Guangdong Ocean University, Zhanjiang 524088, Guangdong Province, China;Life Science and Technology School of Zhanjiang Normal University, Zhanjiang 524048, Guangdong Province, China;Agricultural Institute of Guangdong Ocean University, Zhanjiang 524088, Guangdong Province, China |
| Abstract: | The research was conducted to reveal the regular of resistance to sheath blight and chitinase activities of 13 transgenic rice lines which modified by chitinase gene and other anti-fungal genes. The results showed that the resistances of all transgenic rice lines were higher than their mothers. There were high resistant transgenic lines in which derived from different resistant mothers and modified by single antifungual gene or multiple antifungal genes. The disease-index variation coefficients of transgenic rice lines were 21.93-69.32, wider than the non-transgenic ones, 14.37-47.00. It indicated that resistance of the transgenic rice changed more easily. The analysis of activity of chitinases indicated that the chitinase activities of transgenic rice were higher than their mothers, except one line. But there were no close relationship between resistance and the enzyme activity in transgenic rice lines. |
| Keywords: | transgenic rice sheath blight resistance chitinase |
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