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玫瑰黄链霉菌Men-myco-93-63活性代谢产物roflamycoin的光稳定性分析
引用本文:刘亚南,韩兴,甄丹妹,赤国彤,李亚宁,刘大群.玫瑰黄链霉菌Men-myco-93-63活性代谢产物roflamycoin的光稳定性分析[J].植物保护学报,2018,45(6):1260-1266.
作者姓名:刘亚南  韩兴  甄丹妹  赤国彤  李亚宁  刘大群
作者单位:河北农业大学植物保护学院, 河北省农作物病虫害生物防治工程技术研究中心, 国家北方山区农业工程技术研究中心, 保定 071000;河北德瑞化工有限责任公司, 石家庄 052160,中国农业科学院研究生院, 北京 100081,河北农业大学植物保护学院, 河北省农作物病虫害生物防治工程技术研究中心, 国家北方山区农业工程技术研究中心, 保定 071000,河北农业大学植物保护学院, 河北省农作物病虫害生物防治工程技术研究中心, 国家北方山区农业工程技术研究中心, 保定 071000,河北农业大学植物保护学院, 河北省农作物病虫害生物防治工程技术研究中心, 国家北方山区农业工程技术研究中心, 保定 071000,河北农业大学植物保护学院, 河北省农作物病虫害生物防治工程技术研究中心, 国家北方山区农业工程技术研究中心, 保定 071000;中国农业科学院研究生院, 北京 100081
基金项目:国家重点研发计划(2017YFD0201100),河北省二期农业产业技术体系创新团队项目(HBCT2018060204),中国农业科学院农科英才项目(2017-2018)
摘    要:为研究影响玫瑰黄链霉菌Streptomyces roseoflavus Men-myco-93-63活性代谢产物roflamycoin光不稳定的因素,采用高效液相色谱仪测定了不同光照环境、光照强度、光照时间、溶剂、质量浓度等因素下roflamycoin的光分解率;检测8种光稳定剂对roflamycoin光分解的抑制作用;并通过抑菌圈法测定了光分解前后物质抑菌效果的变化。结果显示,在roflamycoin的光分解过程中,太阳光和日光灯是其敏感光源,而紫外光并不能引起光分解;光照强度越大、光照时间越长、质量浓度越低,其光分解速率越快;在甲醇、丙酮、N,N-二甲基甲酰胺、二甲基亚砜、四氢呋喃5种溶剂中的光分解速率无显著差异;roflamycoin光分解过程最终可以达到一个稳定状态,主要分解为3种物质,并与roflamycoin具有相同的五烯大环内酯骨架;8种光稳定剂均未表现出抑制光分解的作用。roflamycoin光照分解后对12种病原菌仍有抑制作用,其中对番茄灰霉病菌、玉米穗腐病菌等6种病原菌的抑制活性显著增强,抑菌圈直径增大7.64%~21.91%,对棉花黄萎病菌、苹果斑点落叶病菌等4种病原菌的抑制活性显著降低,抑菌圈直径减小10.03%~91.46%。推测roflamycoin的光分解产物仍具有抑菌活性。

关 键 词:玫瑰黄链霉菌Men-myco-93-63  roflamycoin  光分解  生物活性
收稿时间:2017/7/11 0:00:00

Photolysis of the active metabolite roflamycoin produced by Streptomyces roseoflavus Men-myco-93-63
Liu Yanan,Han Xing,Zhen Danmei,Chi Guotong,Li Yaning and Liu Daqun.Photolysis of the active metabolite roflamycoin produced by Streptomyces roseoflavus Men-myco-93-63[J].Acta Phytophylacica Sinica,2018,45(6):1260-1266.
Authors:Liu Yanan  Han Xing  Zhen Danmei  Chi Guotong  Li Yaning and Liu Daqun
Institution:Hebei Provincial Biological Control Center of Plant Diseases and Plant Pests, National Engineering Research Center for Agriculture in Northern Mountainous Areas, College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China;Hebei Derui Chemical Co. Ltd., Shijiazhuang 052160, Hebei Province, China,Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China,Hebei Provincial Biological Control Center of Plant Diseases and Plant Pests, National Engineering Research Center for Agriculture in Northern Mountainous Areas, College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China,Hebei Provincial Biological Control Center of Plant Diseases and Plant Pests, National Engineering Research Center for Agriculture in Northern Mountainous Areas, College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China,Hebei Provincial Biological Control Center of Plant Diseases and Plant Pests, National Engineering Research Center for Agriculture in Northern Mountainous Areas, College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China and Hebei Provincial Biological Control Center of Plant Diseases and Plant Pests, National Engineering Research Center for Agriculture in Northern Mountainous Areas, College of Plant Protection, Hebei Agricultural University, Baoding 071000, Hebei Province, China;Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Abstract:To determine the factors that affect the light instability of the active metabolite roflamycoin, the photolysis rate under different light sources, light intensities, illumination time, solvents and antibiotic concentrations were tested by high performance liquid chromatography (HPLC). The inhibition of photodegradation was performed by using eight kinds of light stabilizers such as UV absorbers, antioxidants and so on. The antifungal effect of the substances before and after photodegradation was detected by inhibition zone method. The results showed that it was sensitive under the sunlight and fluorescent light, while UV-light could not result in photolysis. The greater the illumination intensity, and the longer the illumination time, the lower the mass concentration, and the faster the photolysis rates. No significant differences were found in the photolysis rates when roflamycoin was dissolved in methanol, acetone, N, N-dimethyl formamide, dimethyl sulfoxide or tetrahydrofuran. Finally it decomposed into three compounds with pentaene macrolide (same with roflamycoin) and the photolysis process reached a balance. The eight kinds of light stabilizers all showed no inhibition effects on photodegradation. The roflamycoin after photolysis could still provide effective inhibition against 12 kinds of plant pathogens, and significantly increased its inhibition activities by 7.64%-21.91% against six pathogens, including Botrytis cinerea and Fusarium verticillioides, while decreased its inhibition activities by 10.03%-21.91% against the other four pathogens, including Verticillium dahilae and Alternaria brassicae. The results indicated that the photolysis did not change the biological activity of roflamycoin.
Keywords:Streptomyces roseoflavus Men-myco-93-63  roflamycoin  photolysis  biological activity
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