| 我国甘蔗主要杂交亲本黄叶病病原鉴定及田间发病率 |
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| 引用本文： | 高三基,林彦铨,邓祖湖,徐良年,陈如凯.我国甘蔗主要杂交亲本黄叶病病原鉴定及田间发病率[J].植物保护学报,2011,38(3):240-246. |
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| 作者姓名： | 高三基 林彦铨 邓祖湖 徐良年 陈如凯 |
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| 作者单位： | 福建农林大学，农业部甘蔗遗传改良重点开放实验室，福州350002 |
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| 基金项目： | 现代农业产业技术体系建设专项(nycytx-024),公益性行业(农业)科研专项(nyhyzx07-019),福建省自然科学基金(2008J0048) |
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| 摘 要： | 甘蔗黄叶病是一种发生普遍、危害严重的病毒病害,选育抗病品种是控制该病发生与蔓延的有效措施.利用逆转录-聚合酶链反应(RT-PCR)、组织印迹杂交免疫测定(TBIA)和抗原直接包被酶联免疫吸附(DAC-ELISA)方法对我国43份甘蔗主要杂交亲本黄叶病病原(Sugarcane yellow leaf virus,SCYLV)进行鉴定,并调查SCYLV田间自然侵染条件下甘蔗黄叶病发病率.已明确36份亲本材料感染或未感染SCYLV,其中32份鉴定结果为阳性,品种(系)感病率达88.9％.甘蔗植株田间自然发病率可分为无发病、发病率低、中等和高4个等级,方差分析表明,各等级间的植株发病率差异达极显著水平(P＜0.01).从美国引进的多数CP和HoCP系列亲本发病率较高,而多数崖城和新台糖系列亲本材料的发病率较低,可作为甘蔗抗黄叶病杂交亲本.
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| 关 键 词： | 甘蔗黄叶病毒 病原鉴定 血清学反应 杂交育种 抗病性 |
| 收稿时间： | 2010/8/16 0:00:00 |
Pathogenic identification of sugarcane yellow leaf disease and incidence of disease-infected plant in main sugarcane parental clones in China |
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| Gao Sanji,Lin Yanquan,Deng Zuhu,Xu Liangnian and Chen Rukai.Pathogenic identification of sugarcane yellow leaf disease and incidence of disease-infected plant in main sugarcane parental clones in China[J].Acta Phytophylacica Sinica,2011,38(3):240-246. |
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| Authors: | Gao Sanji Lin Yanquan Deng Zuhu Xu Liangnian and Chen Rukai |
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| Institution: | Fujian Agriculture and Forestry University, Key Laboratory of Sugarcane Genetic Improvement, Ministry of Agriculture, Fuzhou 350002, Fujian Province, China;Fujian Agriculture and Forestry University, Key Laboratory of Sugarcane Genetic Improvement, Ministry of Agriculture, Fuzhou 350002, Fujian Province, China;Fujian Agriculture and Forestry University, Key Laboratory of Sugarcane Genetic Improvement, Ministry of Agriculture, Fuzhou 350002, Fujian Province, China;Fujian Agriculture and Forestry University, Key Laboratory of Sugarcane Genetic Improvement, Ministry of Agriculture, Fuzhou 350002, Fujian Province, China;Fujian Agriculture and Forestry University, Key Laboratory of Sugarcane Genetic Improvement, Ministry of Agriculture, Fuzhou 350002, Fujian Province, China |
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| Abstract: | |
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| Keywords: | Sugarcane yellow leaf virus pathogenic identification serologic reactions hybrid breeding disease resistance |
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