褐飞虱共生菌抗感吡虫啉菌株体内抗氧化酶活性的比较

在含不同浓度吡虫啉的综合马铃薯葡萄糖琼脂液体培养基中接种抗感吡虫啉褐飞虱共生菌菌株且培养2～6天后,通过测定抗吡虫啉菌株和敏感菌株的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)等活性,阐明抗感菌株在吡虫啉胁迫下体内抗氧化酶活性的变化.结果显示,抗吡虫啉菌株和敏感菌株三种抗氧化酶活性差异明显不同,抗性菌株的SOD、POD活性(极)显著高于敏感菌株,其中抗性菌株SOD活性比敏感菌株提高了45.89％～222.53％；抗性菌株的CAT活性与敏感菌株差异无明显规律性.说明褐飞虱共生菌对吡虫啉的抗药性与其体内SOD和POD的活性增强有关.

Comparison for activities of antioxidant enzymes in imidacloprid resistant-and susceptible symbiotic strains in rice brown planthopper, Nilaparvata lugens (Stål)

After imidacloprid resistant-and susceptible symbiotic strains of rice brown planthopper, Nilaparvata lugens were cultured in CPDA (comprehensive potato dextrose agar) liquid culture medium containing different concentrations of imidacloprid for two to six days, activities of antioxidant enzymes (superoxide dismutase, catalase, peroxidase) in imidacloprid resistant-and susceptible strains were determined, and change of these enzymes activities in different imidacloprid resistant strains were elucidated. The results showed that imidacloprid resistant-and susceptible strains had significant difference in activities of superoxide dismutase, peroxidase and catalase. The activities of superoxide dismutase and peroxidase in imidacloprid resistant strains were significantly higher than those in imidacloprid susceptible strains, and activities of superoxide dismutase in imidacloprid resistant strains had increased 45.89%-222.53%, while change of catalase activity had no obvious regularity in imidacloprid resistant-and susceptible strains. It was suggested that resistant of N.lugens symbiotes to imidacloprid was related to enh-ancement of superoxide dismutase and peroxidase activities.