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| 枯草芽胞杆菌对离体荔枝果实霜疫霉病、炭疽病的防治效果 | |
| 引用本文： | 黄庶识,黄曦,张荣灿,许兰兰,雷富,黄荣韶.枯草芽胞杆菌对离体荔枝果实霜疫霉病、炭疽病的防治效果[J].植物保护学报,2011,38(3):247-252. |
| 作者姓名： | 黄庶识 黄曦 张荣灿 许兰兰 雷富 黄荣韶 |
| 作者单位： | 1. 广西科学院生物物理实验室，南宁530007;昆明理工大学生物工程技术研究中心，昆明650224 2. 广西科学院生物物理实验室，南宁530007;广西大学农学院，南宁530004 3. 广西科学院海洋环境监测中心,南宁,530007 4. 广西大学农学院,南宁,530004 |
| 基金项目： | 国家自然科学基金(30860010),广西科学基金(桂科回0832006),广西科研院所基本科研业务费资助项目(08YJ16WL01) |
| 摘 要： | 为了进一步明确从土壤中筛选得到的拮抗细菌OR-1、OR-2、OR-3、ON-6的分类地位和生防效果,采用形态学观察、理化特性结合分子生物学方法,鉴定这4株细菌皆为枯草芽胞杆菌Bacillussubitilis.拮抗菌对荔枝霜疫霉菌、炭疽菌菌丝生长均具有明显抑制作用,ON-6菌株的发酵液对菌丝生长的抑制率最高,分别为92.34％和70.36％,其次是OR-1菌株.拮抗菌处理组褐变指数均小于对照组以及杀菌剂处理组,且差异达显著水平(P＜0.05),OR-1菌株防褐变效果最好.常温下,拮抗菌发酵液对离体鲜果病害防治效果优于对照组和杀菌剂处理组,其中ON-6菌株的防治效果最佳；4℃低温储藏处理40天后,拮抗菌发酵液处理组的防治效果高于对照和杀菌剂处理组,且差异显著(P＜0.05),以ON-6和ON-3菌株的防治效果最佳.表明拮抗菌发酵液对荔枝鲜果上的霜疫霉菌和炭疽病菌有一定的抑制作用. |
| 关 键 词： | 枯草芽胞杆菌 荔枝 霜疫霉菌 炭疽病菌 拮抗作用 |
| 收稿时间： | 8/5/2010 12:00:00 AM |
The effects of biological control of Bacillus subtilis strains on downy mildew and anthracnose diseases of harvested litchi fruits |
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| Huang Shushi,Huang Xi,Zhang Rongcan,Xu Lanlan,Lei Fu and Huang Rongshao.The effects of biological control of Bacillus subtilis strains on downy mildew and anthracnose diseases of harvested litchi fruits[J].Acta Phytophylacica Sinica,2011,38(3):247-252. | |
| Authors: | Huang Shushi Huang Xi Zhang Rongcan Xu Lanlan Lei Fu and Huang Rongshao |
| Institution: | Biophysics Laboratory, Guangxi Academy of Sciences, Nanning 530007, Guangxi Zhuang Autonomous Region, China;Biotechnology Research Center, Kunming University of Science and Technology, Kunming 650224, Yunnan Province, China;Biophysics Laboratory, Guangxi Academy of Sciences, Nanning 530007, Guangxi Zhuang Autonomous Region, China;Agricultural College, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous Region, China;Marine Environment Monitoring Center, Guangxi Academy of Sciences, Nanning 530007, Guangxi Zhuang Autonomous Region, China;Biophysics Laboratory, Guangxi Academy of Sciences, Nanning 530007, Guangxi Zhuang Autonomous Region, China;Biotechnology Research Center, Kunming University of Science and Technology, Kunming 650224, Yunnan Province, China;Marine Environment Monitoring Center, Guangxi Academy of Sciences, Nanning 530007, Guangxi Zhuang Autonomous Region, China;Agricultural College, Guangxi University, Nanning 530004, Guangxi Zhuang Autonomous Region, China |
| Abstract: | Four antagonistic bacteria strains,OR-1,OR-2,OR-3 and ON-6,which were isolated and screened from soil under litchi trees and identified as Bacillus subtilis morphologically and molecularly,were used to evaluate the control effect on downy mildew and anthracnose diseases of harvested litchi fruits.The results showed that all 4 strains had obvious inhibiting action on mycelial growth of Peronophythora litchii and Colletrichum gloeosporiodes,among them,ON-6 strain had the highest inhibitory rate to mycelial gr... |
| Keywords: | Bacillus subtilis Litchi chinensis Peronophythora litchi Colletotrichum gloeosporioides antagonistic |
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