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| 抗青枯病转多肽抗生素基因烟草的选育 | |
| 引用本文： | 徐进,冯洁,顾刚,陈顺辉,彭学贤,孙超.抗青枯病转多肽抗生素基因烟草的选育[J].植物保护学报,2008,35(2):102-106. |
| 作者姓名： | 徐进 冯洁 顾刚 陈顺辉 彭学贤 孙超 |
| 作者单位： | 1. 中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京,100094 2. 福建省烟草农业科学研究所,福州,350003 3. 中国科学院微生物研究所,北京,100080 |
| 基金项目： | 国家烟草专卖局资助项目 , 国家自然科学基金 |
| 摘 要： | 分别于温室和田间条件下,对转单价apidaecin、shiva-I及双价apidaecin+shiva-I基因的14个烟草株系材料的T1和T2后代群体进行青枯病抗性筛选鉴定及农艺性状评价。温室盆栽试验中,于5~6叶龄期采用伤根法进行接种。接种后15天,转apidaecin、shiva-I和apidaecin+shiva-I基因烟草的病情指数较对照分别下降了16.3%、15.2%和59.7%。田间自然病圃筛选鉴定结果表明:B43-5-4、B43-5-1、B43-5-2、B43-5-3和B43-4-1等5个株系材料病情指数较起始品种K326降低21%~70%,且农艺性状与起始品种K326无显著差异。 |
| 关 键 词： | 多肽抗生素 转基因烟草 青枯病 抗病性 抗青枯病 多肽抗生素 基因 烟草 选育 bacterial wilt genes antibiotic peptide transgenic tobacco 差异 农艺性状 品种 鉴定结果 病圃筛选 田间 病情指数 接种 伤根法 叶龄 |
| 收稿时间： | 2008/1/18 0:00:00 |
| 修稿时间： | 2008/1/18 0:00:00 |
The breeding of transgenic tobacco with peptide antibiotic genes against bacterial wilt |
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| Xu Jin,Feng Jie,Gu Gang,Chen Shunhui,Peng Xuexian and Sun Chao.The breeding of transgenic tobacco with peptide antibiotic genes against bacterial wilt[J].Acta Phytophylacica Sinica,2008,35(2):102-106. | |
| Authors: | Xu Jin Feng Jie Gu Gang Chen Shunhui Peng Xuexian and Sun Chao |
| Institution: | State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094,China;State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094,China;Fujian Academy of Tobacco Sciences, Fuzhou 350003, Fujian Province, China;Fujian Academy of Tobacco Sciences, Fuzhou 350003, Fujian Province, China;Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China;Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China |
| Abstract: | 14 T1 and T2 transgenic tobacco lines with apidaecin, shiva-I and apidaecin+shiva-I genes were evaluated for bacterial wilt resistance and agronomic trait both in greenhouse and field plot, 15 days after inoculation with R.solanacearum under greenhouse conditions. All three kinds of transgenic plants showed enhanced resistance. The transgenic plants harboring apidaecin+shiva-I cassette showed the highest disease resistance among all three groups of transgenic plants. Five T2 transgenic lines (B43-5-4, B43-5-1, B43-5-2, B43-5-3 and B43-4-1) decreased disease indices by 21%-70% in field trial. In the meantime, they exhibited no significant difference in agronomic traits and quality by comparing with original cultivar K326. |
| Keywords: | peptide antibiotic transgenic tobacco bacterial wilt resistance |
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