丹皮酚对植物病原真菌的体外抑制作用

为揭示丹皮酚在牡丹体内的抗菌机制,采用生长速率法测定了丹皮酚对供试病菌菌丝生长的抑制作用;用涂布平板法测定了丹皮酚对供试病菌孢子萌发及形成的影响。结果表明,丹皮酚含量与病菌菌落直径线性增长及菌丝生物学产量的相对抑制率呈正相关,在丹皮酚含量0.08、0.4mg/mL培养液中培养禾谷丝核菌和茄病镰刀菌5天,对菌丝干重的抑制率分别为79.81%和55.08%。丹皮酚对供试病菌的孢子萌发无作用,但孢子萌发后,其芽管基部膨大、顶端膨大及中间局部膨大。丹皮酚推迟病菌分生孢子梗的形成时间,降低分生孢子的形成数量,在0.20、0.24mg/mLPSA培养基上培养玉蜀黍赤霉和茄病镰刀菌10天,对分生孢子形成的抑制率分别为100%和33.33%。

Inhibition activities of paeonol to plant pathogenic fungi in vitro

In order to reveal the antibacterial mechanism of paeonol in the peony, the inhibition of mycelium growth was measured by the growth rate and the effects of spore germination and formation were measured by coating plate in tests. The results showed that paeonol content to the relative inhibiting rate of the linear growth of fungal colony diameter and mycelia biomass yield was positively correlated, when Rhizoctonia cerealis and Fusarium solani were cultured for five days in paeonol content 0.08, 0.4 mg/mL medium culture, the inhibiting rates of dry weight of mycelium were 79.81% and 55.08%. Paeonol had no effects on germinating rate for the spores, but germ tubes became swollen at the apical, basal and middle. Paeonol delay the time of formation for conidiophores and reduce the number of the spores. Gibberella zeae and Fusarium solani were cultured for ten days in 0.20, 0.24 mg/mL PSA medium, the inhibiting rates of the spores were 100% and 33.33%.