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两个小麦-簇毛麦易位系抗条锈基因的遗传分析和SSR标记检测
引用本文:尹军良,王睿,唐明双,马东方,王海鸽,井金学.两个小麦-簇毛麦易位系抗条锈基因的遗传分析和SSR标记检测[J].植物保护学报,2015,42(2):153-159.
作者姓名:尹军良  王睿  唐明双  马东方  王海鸽  井金学
作者单位:西北农林科技大学植物保护学院, 旱区作物逆境生物学国家重点实验室, 陕西 杨凌 712100;中国农业大学开封实验站, 河南 开封 475004;南充市农业科学院, 四川 南充 637000;长江大学农学院, 湖北 荆州 434025;西北农林科技大学植物保护学院, 旱区作物逆境生物学国家重点实验室, 陕西 杨凌 712100;西北农林科技大学植物保护学院, 旱区作物逆境生物学国家重点实验室, 陕西 杨凌 712100
基金项目:高等学校学科创新引智计划(B07049), 国家公益性行业(农业)科研专项(2009003035)
摘    要:为明确普通小麦-簇毛麦易位系材料对不同条锈菌系的抗病水平、抗病基因组成和易位系间抗病基因关系,对V9125-3和V9125-4易位系进行了苗期抗条锈性遗传分析,并利用V9125-2抗条锈基因Yr WV的2个侧翼分子标记,分析了3个易位系抗病基因间的关系。结果表明,2个易位系对当前国内7个优势菌系均表现良好的抗病性,但对不同菌系抗病性的抗病基因遗传特点有所不同。V9125-3对CYR29、CYR30和CYR31的抗病性由2对显性基因独立控制,对CYR32、CYR33和Sun11-11的抗病性由1显1隐2对基因控制,对Sun11-4的抗病性由2对显性基因互补控制;V9125-4对CYR30、Sun11-4和Sun11-11的抗病性由2对显性基因独立控制,对CYR32和CYR33的抗病性由1显1隐2对基因控制,对CYR29和CYR31的抗病性由2对显性基因互补控制;V9125-3对CYR29的抗病基因其中之一可能是Yr WV,另一个为未知基因。

关 键 词:簇毛麦  易位系  小麦条锈病  遗传分析  标记检测
收稿时间:3/6/2014 12:00:00 AM

Genetic and SSR molecular marker associate analysis of two translocation lines from Triticum aestivum-Haynaldia villosa resistant to stripe rust
Yin Junliang,Wang Rui,Tang Mingshuang,Ma Dongfang,Wang Haige and Jing Jinxue.Genetic and SSR molecular marker associate analysis of two translocation lines from Triticum aestivum-Haynaldia villosa resistant to stripe rust[J].Acta Phytophylacica Sinica,2015,42(2):153-159.
Authors:Yin Junliang  Wang Rui  Tang Mingshuang  Ma Dongfang  Wang Haige and Jing Jinxue
Institution:State Key Laboratory for Crop Stress Biology in Arid Areas, College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi Province, China;Kaifeng Experimental Station of China Agricultural University, Kaifeng 475004, Henan Province, China;Nanchong Academy of Agricultural Science, Nanchong 637000, Sichuan Province, China;College of Agriculture, Yangtze University, Jingzhou 434025, Hubei Province, China;State Key Laboratory for Crop Stress Biology in Arid Areas, College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi Province, China;State Key Laboratory for Crop Stress Biology in Arid Areas, College of Plant Protection, Northwest A & F University, Yangling 712100, Shaanxi Province, China
Abstract:For assessing the resistance level of translocation lines to different Puccinia striiformis f.sp. tritici races and dissecting the organization of resistance genes and their relationships among different lines, the stripe rust resistance inheritance characteristics of two translocation lines at seedling stage were analyzed. Using two flanking markers of the resistance gene YrWV from translocation line V9125-2, the relationships between the resistant genes in three translocation lines were analyzed. The results revealed that two translocation lines were high resistant to seven Chinese dominant races of stripe rust and resistant genes contained by these two lines to different races were distinguished in quantity and trait. In V9125-3, resistance to CYR29, CYR30 and CYR31 is controlled by two dominant genes, to CYR32, CYR33 and Sun11-11 controlled by one dominant gene and one recessive gene, to Sun11-4 controlled by complementation of two dominant genes; in V9125-4, resistance to CYR30, Sun11-4 and Sun11-11 are controlled by two dominant genes, to CYR32, CYR33 controlled by one dominant gene and one recessive gene, to CYR29 and CYR31 controlled by complementation of two dominant genes. Genetic analysis data associated with molecular marker tests showed that one of the two resistant genes which control resistance of V9125-3 to CYR29 may be YrWV, another gene is unknown.
Keywords:Haynaldia villosa  translocation line  wheat stripe rust  genetic analysis  markers detection
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