| 高效、准确的玉米粗缩病人工接种鉴定技术 |
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| 引用本文： | 张爱红,邸垫平,张晓芳,路银贵,田兰芝,苗洪芹.高效、准确的玉米粗缩病人工接种鉴定技术[J].植物保护学报,2015,42(1):87-92. |
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| 作者姓名： | 张爱红 邸垫平 张晓芳 路银贵 田兰芝 苗洪芹 |
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| 作者单位： | 河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000;河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000;河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000;河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000;河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000;河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000 |
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| 基金项目： | 国家公益性行业(农业)科研专项(201003031),转基因生物新品种培育科技重大专项(2011ZX08003-001),河北省农林科学院青年基金(A2012120104) |
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| 摘 要： | 为建立高效、准确的玉米粗缩病人工接种鉴定技术,通过将玉米人工接种水稻黑条矮缩病毒(Rice black-streaked dwarf virus,RBSDV),研究了温度对RBSDV在灰飞虱体内循回期的影响以及玉米接种苗龄、接种强度及接种时间对人工接种鉴定效果的影响。结果表明:病毒在灰飞虱体内的循回期随温度升高而缩短,在平均温度分别为18.50、23.50、27.50℃时,循回期分别为36.27、21.76、16.38 d;在玉米芽鞘期至2叶1心期接种,接种强度每苗为7~10头虫,接种时间72~96 h条件下,鉴定效果优于其它处理;感病对照郑单958、掖107、掖478发病充分,病情指数为97.70%~100.00%。用已知抗性水平的玉米自交系和杂交种进行验证,鉴定结果表明该人工接种鉴定技术可客观反映供试玉米材料的实际抗性水平。
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| 关 键 词： | 玉米粗缩病 水稻黑条矮缩病毒 人工接种 鉴定 |
| 收稿时间： | 2014/1/14 0:00:00 |
Efficient and accurate artificial inoculation technique of Rice black-streaked dwarf virus to maize |
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| Zhang Aihong,Di Dianping,Zhang Xiaofang,Lu Yingui,Tian Lanzhi and Miao Hongqin.Efficient and accurate artificial inoculation technique of Rice black-streaked dwarf virus to maize[J].Acta Phytophylacica Sinica,2015,42(1):87-92. |
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| Authors: | Zhang Aihong Di Dianping Zhang Xiaofang Lu Yingui Tian Lanzhi and Miao Hongqin |
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| Institution: | Integrated Pest Management Center of Hebei Province; Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture; Plant Protection Institute of Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China;Integrated Pest Management Center of Hebei Province; Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture; Plant Protection Institute of Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China;Integrated Pest Management Center of Hebei Province; Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture; Plant Protection Institute of Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China;Integrated Pest Management Center of Hebei Province; Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture; Plant Protection Institute of Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China;Integrated Pest Management Center of Hebei Province; Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture; Plant Protection Institute of Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China;Integrated Pest Management Center of Hebei Province; Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture; Plant Protection Institute of Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China |
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| Abstract: | |
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| Keywords: | maize rough dwarf disease Rice black-streaked dwarf virus artificial inoculation method identification |
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