中国疫区内苹果蠹蛾微卫星位点的扩增稳定性及遗传多样性

为揭示欧洲苹果蠹蛾微卫星位点在中国苹果蠹蛾群体遗传学研究中的有效性,以12对微卫星引物对采自中国主要疫区新疆、甘肃、黑龙江的8个苹果蠹蛾地理种群的120头个体在各位点的遗传多样性及扩增稳定性进行研究。所选取的12个微卫星位点中,有8个能够稳定扩增,各位点在大多数种群中均显示偏离哈迪-温伯格平衡,各位点上的平均等位基因数量为3.750～12.500,平均观察杂合度Ho为0.025～0.783,平均期望杂合度He为0.284～0.892。位点Cp2.P和Cp4.56分别具有较低的观察杂合度(0.109、0.025)和较高的近交系数Fis(0.806、0.954),说明这2个位点上的杂合子非常缺乏,其余6个位点均具有较高的杂合度水平和等位基因数量,适用于中国疫区内苹果蠹蛾微卫星分子标记研究。

Amplifying stability and genetic diversity of microsatellite loci in codling moth Cydia pomonella (Lepidoptera: Tortricidae) populations in China

In order to find out whether the microsatellite loci screened from European Cydia pomonella populations are available for microsatellite markers in Chinese C.pomonella populations, the amplifying stability and genetic diversity of 12 microsatellite loci were investigated based on eight geographic populations of codling moth including 120 individuals collected from mainly distributed regions of China. It was observed that eight of the 12 selected loci could be successfully amplified through polymerase chain reaction (PCR). Most populations revealed departure from Hardy-Weinberg equilibrium and the mean number of alleles ranged from 3.750 to 12.500 at each loci. The mean value of observed heterozygosity (Ho) was between 0.025 and 0.783, and the excepted heterozygosity (He) was between 0.284 and 0.892. The lowest value of observed heterozygosity and highest value of inbreeding coefficient (Fis) were observed at locus Cp4.56 (Ho: 0.025; Fis: 0.954), followed by Cp2.P (Ho: 0.109; Fis: 0.806), sug- gesting significant heterozygote deficiencies at these two loci. The other six microsatellite loci showed re- latively higher heterozygosity and larger amount of alleles and thus could be considered usable in research of microsatellite markers for Chinese C.pomonella populations.