四川省籼稻区水稻稻曲病菌遗传多样性分析

应用ERIC-PCR指纹技术,对采自四川省6个籼稻自然生态区的60个稻曲病菌菌株进行了DNA分子水平上的遗传多样性研究,并进行UPGMA聚类分析和相似性分析.结果表明,所采用的ERIC引物在不同供试菌株中分别扩增出5～20条不等的带谱;在0.75相似水平上,供试菌株被划分为11个遗传型群,其中L1、L4、L5为优势群,并存在次要小型群和特异性型群;来自同一地区的稻曲病菌菌株具有较高的遗传相似性,而不同地区的稻曲病菌则表现出程度不同的变异,且寄主品种与稻曲病菌遗传差异之间的相关性较小.

Preliminary analysis of genetic diversity of Ustilaginoidea virens strains of indica rice from Sichuan Province

In the present study, the genetic diversity of the 60 Ustilaginoidea virens isolates, which were collected from 6 indica rice production regions in Sichuan Province, have been investigated with ERIC-PCR (enterobacterial repetitive intergenic consensus-polymerase chain reaction). Using the ERIC primers, the polymorphic bands ranging from 5 to 20 of each examined isolate were detected. According to cluster analysis of UPGMA, 11 clusters (L1 to L11) were formed based on the boundary level of 0.75 average distance; and clusters of L1, L4 and L5 were predominant groups and there also exist some subordinate and special groups. The results of similarity analysis indicated that the U.virens isolates collected from the similar regions possessed the considerable stability in DNA composition, while the isolates which collected from the different regions showed some regionalism in different degrees; and there exist low degree of correlation between rice varieties and genetic diversity of the different strains.