二氯喹啉酸诱导烟草过氧化物酶 活性的动态分析

为了验证不同剂量二氯喹啉酸致烟草畸形生长与诱导植物体内保护酶系中的过氧化物酶活性间的关系,采用盆栽试验的方法,通过添加二氯喹啉酸,模拟田间二氯喹啉酸在土壤中的不同残留量,造成烟草不同程度的畸形生长,在不同时间内,测定烟叶中过氧化物酶(POD)活性的变化。结果表明,处理组烟叶中POD酶的活性明显高于未用药的对照烟叶,随着处理浓度的升高和时间的延长,POD酶的活性有上升趋势,当处理28天后,各处理的二氯喹啉酸浓度为1.04×10-3、2.08×10-3、4.17×10-3、8.33×10-3和1.67×10-2mg/kg,对照和各处理的过氧化物酶活力分别为17.47、29.61、23.73、30.33、41.00、57.73OD·g-1·min-1,烟叶中过氧化物酶活性较对照分别提高69.47%、35.80%、73.61%、134.69%和230.45%。POD同工酶的检测表明,处理过的烟叶酶带增多、颜色加重、酶活增强。

Dynamic analysis of peroxidase activity in tobacco leaf induced by quinclorac

In order to explain the relationship between the deformity of tobacco caused by quinclorac and the activity of peroxidase, a protective enzyme in the plant induced by the chemical, modeling the dose of quinclorac residue in soil, the seedling of tobacco was transplanted to the soil mixed with quinclorac of different concentrations. The deforming of tobacco growth appeared in different degrees after 7 d. Peroxidase (POD) was studied between deformity and normality of tobacco. The results showed that the activity of POD in tobacco leaf treated with quinclorac was obviously higher than that of the control and the activity increased continually with the increasing of concentration in 35 days. When treated with 1.04×10^-3,2.08×10^-3, 4.17×10^-3 , 8.33×10^-3 and 1.67×10^-2 mg/kg, the specific activity of POD was 29.61, 23.73, 30.33, 41.00 and 57.73 OD · g^-1 · min^-1 respectively after 28 days; whereas that of the CK was 17.47 OD · g^-1 · min^-1. The rate of activation of POD was 69.47%, 35.80%, 73.61%, 134.69%, 230.45%, respectively. The assay of isoenzymes of POD showed that the enzyme band of deformed leaf treated with quinclorac was more than that of the control , and the color of enzyme band of the deformed leaf was stronger than the control. The relationship of the activity of POD and the growth deformation of tobacco caused by quinclorac was discussed.