| 新疆加工番茄腐霉根腐病病原鉴定及其rDNA的ITS区段分析 |
| |
| 引用本文： | 赵思峰,方许阳,姜海荣,杜娟,李春.新疆加工番茄腐霉根腐病病原鉴定及其rDNA的ITS区段分析[J].植物保护学报,2009,36(3):219-224. |
| |
| 作者姓名： | 赵思峰 方许阳 姜海荣 杜娟 李春 |
| |
| 作者单位： | 1. 石河子大学绿洲农作物病害防控重点实验室,新疆石河子,832003
2. 石河子大学绿洲农作物病害防控重点实验室,新疆石河子,832003;北京理工大学生命科学院,北京,100081 |
| |
| 基金项目： | 新疆兵团基础研究计划(ZD2007JC02),新疆高新技术重点项目(20081108),石河子大学成果转化项目(kjkfyzh2007-cgzh01) |
| |
| 摘 要： | 为了明确新疆加工番茄根腐病的病原种类,从成苗期到果实成熟期对各主栽区的病原物进行分离,并进行形态学鉴定和ITS序列分析.结果表明,从162个根腐病样中获得120个分离物,其中腐霉菌93株,占总分离物的77.5%.腐霉菌分离物鉴定为3个种,瓜果腐霉Pythium aphani-dermatum(Edson)Fitzp,56株,占腐霉分离物的60.22%;简囊腐霉P.monospermum Pringsheim,6株,占6.45%;棘腐霉P.acanthicum Drechsler,2株,占2.15%;另有29个分离物因未诱发出雄器和藏卵器而无法鉴定到种.
|
| 关 键 词： | 加工番茄 腐霉菌 根腐病 rDNA-ITS区序列分析 鉴定 |
| 收稿时间： | 2008/8/19 0:00:00 |
Identification and rDNA ITS sequence analysis of the Pythium root rot pathogens of processing tomato in Xinjiang |
| |
| Zhao Sifeng,Fang Xuyang,Jiang Hairong,Du Juan and Li Chun.Identification and rDNA ITS sequence analysis of the Pythium root rot pathogens of processing tomato in Xinjiang[J].Acta Phytophylacica Sinica,2009,36(3):219-224. |
| |
| Authors: | Zhao Sifeng Fang Xuyang Jiang Hairong Du Juan and Li Chun |
| |
| Institution: | The Key Laboratory of Prevention and Control for Oasis Crop Disease, Shihezi University, Shihezi 832003, Xinjiang Uygur Autonomous Region, China;The Key Laboratory of Prevention and Control for Oasis Crop Disease, Shihezi University, Shihezi 832003, Xinjiang Uygur Autonomous Region, China;The Key Laboratory of Prevention and Control for Oasis Crop Disease, Shihezi University, Shihezi 832003, Xinjiang Uygur Autonomous Region, China;The Key Laboratory of Prevention and Control for Oasis Crop Disease, Shihezi University, Shihezi 832003, Xinjiang Uygur Autonomous Region, China;The Key Laboratory of Prevention and Control for Oasis Crop Disease, Shihezi University, Shihezi 832003, Xinjiang Uygur Autonomous Region, China; School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, China |
| |
| Abstract: | In order to investigate the pathogens for instructing agricultural production, the pathogens which caused root rot of processing tomato were isolated from diseased processing tomato from seeding stage to fruit ripening stage in the different main production areas. Pythium isolates were identified according to the morphological features of culture and the ITS sequence analyzed of rDNA of representative strains. The results showed that 162 samples of root rot diseased tomato plant were collected and 120 isolates were obtained. Ninety-three of these, 77.5% of the total isolates, belong to Pythium which was the main pathogen for root rot of processing tomato in Xinjiang. These isolates were identified to class into three different species, Pythium aphanidermatum (Edson) Fitzp, P.monospermum Pringsheim and P.acanthicum Drechsler. P.aphanidermatum is 56 strains, accounted for 60.22%, P.monospermum is 6 strains, accounted for 6.45% and P.acanthicum is 2 strains, accounted for 2.15% in the total Pythium isolates. Other 29 strains of Pythium isolates could not be identified due to no oogonium and sporangia induced. |
| |
| Keywords: | processing tomato Pythium root rot disease rDNA internal transcribed spacer sequence identification |
| 本文献已被 万方数据 等数据库收录！ |
| 点击此处可从《植物保护学报》浏览原始摘要信息 |
| 点击此处可从《植物保护学报》下载免费的PDF全文 |
|
