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| 河南商丘地区棉花黄萎病菌分离鉴定和致病力分析 | |
| 引用本文： | 丁锦平,刘冬梅,周瑞阳,李付广,李成伟.河南商丘地区棉花黄萎病菌分离鉴定和致病力分析[J].植物保护学报,2010,37(4):295-299. |
| 作者姓名： | 丁锦平 刘冬梅 周瑞阳 李付广 李成伟 |
| 作者单位： | 1. 广西大学农学院,南宁,530003;商丘师范学院生命科学系,植物与微生物互作重点实验室,商丘476000 2. 商丘师范学院生命科学系,植物与微生物互作重点实验室,商丘476000 3. 广西大学农学院,南宁,530003 4. 中国农业科学院棉花研究所,农业部棉花遗传改良重点开放实验室,河南,安阳,455004 5. 商丘师范学院生命科学系,植物与微生物互作重点实验室,商丘476000;周口师范学院生命科学系,植物遗传与分子育种重点实验室,周口,466001 |
| 基金项目： | 国家转基因生物新品种培育重大专项子任务(2008ZX08010-004) |
| 摘 要： | 为探讨河南商丘地区棉花黄萎病菌的致病型群体变异,对该地区棉花上分离的8株单孢菌株的菌落形态、显微结构、致病力、ITS序列、系统进化及菌体蛋白等方面进行了研究。结果表明:这些菌株均属于棉花黄萎病菌Verticilliumdahliae;系统进化树显示8株菌株并没有聚在同一进化枝上;8株黄萎菌菌株存在致病力差异,SQ4菌株致病力最强,属于落叶型,而其它致病力较弱的7个菌株属于非落叶型;不同致病力的菌株间蛋白谱带存在差异。 |
| 关 键 词： | 棉花黄萎病菌 ITS序列 系统进化树 菌体蛋白 致病力 |
| 收稿时间： | 2009/12/30 0:00:00 |
Identification and phylogenetic analysis of cotton Verticillium dahliae strains from Shangqiu of Henan Province in China |
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| Ding Jinping,Liu Dongmei,Zhou Ruiyang,Li Fuguang and Li Chengwei.Identification and phylogenetic analysis of cotton Verticillium dahliae strains from Shangqiu of Henan Province in China[J].Acta Phytophylacica Sinica,2010,37(4):295-299. | |
| Authors: | Ding Jinping Liu Dongmei Zhou Ruiyang Li Fuguang and Li Chengwei |
| Institution: | College of Agriculture, Guangxi University, Nanning 530003, Zhuang Autonomous Region of Guangxi, China; Key Laboratory of Plant-Microbe Interactions, Department of Life Sciences, Shangqiu Normal University, Shangqiu 476000, Henan Province, China;Key Laboratory of Plant-Microbe Interactions, Department of Life Sciences, Shangqiu Normal University, Shangqiu 476000, Henan Province, China;College of Agriculture, Guangxi University, Nanning 530003, Zhuang Autonomous Region of Guangxi, China;Key Laboratory of Cotton Genetic Improvement, Ministry of Agriculture,Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455004, Henan Province, China;Key Laboratory of Plant-Microbe Interactions, Department of Life Sciences, Shangqiu Normal University, Shangqiu 476000, Henan Province, China; Key Laboratory of Plant Genetics and Molecular Breeding, Department of Life Sciences, Zhoukou Normal University, Zhoukou 466001, Henan Province, China |
| Abstract: | In order to study the pathotype population variation of cotton Verticillium dahliae in Shangqiu of Henan Province, eight cotton V.dahliae strains from this area were analyzed on colony morphology, microscopic structure, pathogenicity, internal transcribed spacer (ITS) sequence, evolution and total fungal protein. The analysis of colony morphology, microscopic structure and ITS sequence indicated that the eight collected strains belonged to V.dahliae. Phylogenetic tree was constructed based on ITS sequences. It was shown that eight strains have different pathogenicities, SQ4 strain showed higher pathogenicity, which belonged to defoliating type, than the other 7 strains, which belonged to non-defoliating type. The SDS-PAGE analysis of total fungal proteins indicated that the strains with different pathogenicities showed different protein profiles. |
| Keywords: | Verticillium dahliae ITS sequencing phylogenetic tree fungal protein pathogenicity |
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