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阿维菌素和温度胁迫对朱砂叶螨自由基及保护酶活性的影响
引用本文:冯宏祖,刘映红,何林,陆蕊娥,杨大兴.阿维菌素和温度胁迫对朱砂叶螨自由基及保护酶活性的影响[J].植物保护学报,2008,35(6):530-536.
作者姓名:冯宏祖  刘映红  何林  陆蕊娥  杨大兴
作者单位:西南大学植物保护学院重庆市昆虫学及害虫控制工程重点实验室,塔里木大学植物科技学院
摘    要:应用酶活力测定的方法,研究朱砂叶螨经阿维菌素和高(低)温处理后对其体内自由基(O2.-)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性的影响。结果表明,朱砂叶螨经阿维菌素处理后,两种群体内的O2.-含量急剧升高,药后4h抗性种群和敏感种群O2.-含量分别是对照的3.42、3.54倍,同时SOD、CAT活性也明显高于对照;处理组中抗性种群体内O2.-含量低于敏感种群,SOD、CAT活性高于敏感种群。不适宜的高温和低温均能使朱砂叶螨体内的O2.-、SOD、CAT、POD活力升高,敏感种群在42℃的热激下SOD、CAT、POD的活性达到最大,分别是对照的4.25、2.06、1.81倍,与其它温度处理差异显著(P<0.05),其后呈下降趋势,而抗性种群酶活性在实验温度下,始终保持平稳上升的趋势;酶活性变化率分析结果显示,SOD对热胁迫的应激性最强,CAT次之,POD最小,抗性种群三种酶活性的变化率均大于敏感种群。

关 键 词:朱砂叶螨  自由基  超氧化物歧化酶  过氧化氢酶  过氧化物酶
收稿时间:2008/6/27 0:00:00
修稿时间:2008/7/21 0:00:00

Effects of abamectin and temperature stresses on free radicals and protective enzyme activities in Tetranychus cinnabarinus
Feng Hongzu,Liu Yinhong,He Lin,Lu Rui''e,Yang Daxing.Effects of abamectin and temperature stresses on free radicals and protective enzyme activities in Tetranychus cinnabarinus[J].Acta Phytophylacica Sinica,2008,35(6):530-536.
Authors:Feng Hongzu  Liu Yinhong  He Lin  Lu Rui'e  Yang Daxing
Institution:Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China; College of Plant Science Technology, Tarim University, Alar 843300, Xinjiang Uygur Autonomous Region, China;Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China;Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China;Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China;Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China
Abstract:
Keywords:Tetranychus cinnabarinus  free radicals  superoxide dismutase  catalase  peroxidese
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