利用UP-PCR、ISSR和AFLP标记分析玉米丝黑穗病菌遗传多样性

利用UP-PCR、ISSR和AFLP分子标记方法研究了我国主要玉米产区34株玉米丝黑穗病菌的遗传多样性。从供试引物中筛选获得具多态性的UP-PCR引物9个、ISSR引物11个和AFLP引物组合22对,分别扩增出113、72和293条谱带,多态性条带比率分别为91.15%、84.7%和83.27%。聚类分析表明,玉米丝黑穗病菌存在丰富的遗传变异,与地理来源无明显相关性。3种分子标记的遗传相似系数矩阵相关性分析表明,UP-PCR与AFLP具有较高的相关性,相关系数为0.698;UP-PCR与ISSR、ISSR与AFLP的相关系数分别为0.659和0.633。从多态性水平、稳定性和可操作性可以看出,UP-PCR技术更适于分析玉米丝黑穗病菌遗传多样性。此外,UP-PCR、ISSR和AFLP标记划分的类群与鉴别寄主划分的致病类型之间存在一定的相关性,吻合率分别为50.0%、60.0%和47.6%。

Genetic diversity of Sporisorium reilianumby UP-PCR, ISSR and AFLP analysis

Genetic diversity of 34 isolates of Sporisorium reilianum from main corn producing areas in China was analyzed by UP-PCR (universally primed PCR), ISSR (internal simple sequence repeat) and AFLP (amplified fragment length polymorphism). The results showed that 9 primers of UP-PCR, 11 primers of ISSR and 22 primers of AFLP generated 113, 72 and 293 polymorphic bands, accounted for 91.15%, 84.7% and 83.27% of total bands respectively. These molecular markers revealed the genetic diversity of the strains tested, and there was no significant correlation between variation and region. The correlation between UP-PCR and AFLP, which was 0.698, was higher than that between UP-PCR and ISSR, or ISSR and AFLP, which were 0.659 or 0.633 respectively, by the analysis of genetic similarity coefficient matrix from three kinds of molecular markers. The UP-PCR technique was suitable for the genetic diversity analysis of S.reilianum in view of the level of polymorphism, stability and operability. In addition, there was a certain correlation between the groups divided by UP-PCR, ISSR and AFLP molecular markers and the pathogenic types identified by host identification, coincidence rate were 50.0%,60.0%,and 47.6% respectively.