| 土壤强还原消毒过程中产生气体对土传病原菌的抑制作用 |
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| 引用本文： | 黄新琦,刘亮亮,朱睿,张金波,蔡祖聪.土壤强还原消毒过程中产生气体对土传病原菌的抑制作用[J].植物保护学报,2016,43(4):627-633. |
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| 作者姓名： | 黄新琦 刘亮亮 朱睿 张金波 蔡祖聪 |
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| 作者单位： | 1. 南京师范大学地理科学学院,南京210023;南京师范大学,江苏省物质循环与污染控制重点实验室,江苏省地理信息资源开发与利用协同创新中心,南京210023;2. 南京师范大学地理科学学院,南京,210023 |
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| 基金项目： | 国家自然科学基金（41301335），中国博士后科学基金（2014M551622），江苏高校优势学科建设工程 |
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| 摘 要： | 土壤强还原(reductive soil disinfestation,RSD)是一种高效、环保的土壤消毒方法,为明确RSD杀菌机理,采用气相色谱和荧光定量PCR等技术研究RSD过程中产生的气体对土传病原菌的杀菌作用。结果表明:RSD过程中土壤能够产生H_2S和NH_3;当在装有25 g病土的三角瓶中分别加入25 m L的H_2S和NH_3时,土壤中尖孢镰刀菌的数量均显著下降,其杀菌效果分别为90.3%和100.0%;当在病土中加入0.125%(w/w)氨水时,尖孢镰刀菌、茄劳尔氏菌、辣椒疫霉和立枯丝核菌的数量分别下降至对照土壤的1.0%、0.3%、0.1%和0.9%;此外,加入10μL氨水即可显著抑制尖孢镰刀菌菌丝生长和孢子萌发,抑制率分别为52.8%和100.0%。表明RSD过程中产生的H_2S和NH_3对RSD过程的杀菌效果起着一定的作用。
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| 关 键 词： | 氨 硫化氢 尖孢镰刀菌 厌氧发酵 |
| 收稿时间： | 2014/12/18 0:00:00 |
The inhibitory effect of volatile gases produced in reductive soil disinfestation on soil-borne plant pathogens |
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| Huang Xinqi,Liu Liangliang,Zhu Rui,Zhang Jinbo and Cai Zucong.The inhibitory effect of volatile gases produced in reductive soil disinfestation on soil-borne plant pathogens[J].Acta Phytophylacica Sinica,2016,43(4):627-633. |
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| Authors: | Huang Xinqi Liu Liangliang Zhu Rui Zhang Jinbo and Cai Zucong |
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| Institution: | School of Geography Science, Nanjing Normal University, Nanjing 210023, Jiangsu Province, China;Jiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, Nanjing Normal University, Nanjing 210023, Jiangsu Province, China,School of Geography Science, Nanjing Normal University, Nanjing 210023, Jiangsu Province, China,School of Geography Science, Nanjing Normal University, Nanjing 210023, Jiangsu Province, China,School of Geography Science, Nanjing Normal University, Nanjing 210023, Jiangsu Province, China;Jiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, Nanjing Normal University, Nanjing 210023, Jiangsu Province, China and School of Geography Science, Nanjing Normal University, Nanjing 210023, Jiangsu Province, China;Jiangsu Provincial Key Laboratory of Materials Cycling and Pollution Control, Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application, Nanjing Normal University, Nanjing 210023, Jiangsu Province, China |
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| Abstract: | |
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| Keywords: | ammonia hydrogen sulfide Fusarium oxysporum anaerobic fermentation |
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