| 草莓枯萎病菌抗多菌灵突变体ZY-R的生理生化特性研究 |
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| 引用本文： | 姜莉莉,顾春波,左一鸣,王开运,段海明.草莓枯萎病菌抗多菌灵突变体ZY-R的生理生化特性研究[J].农药学学报,2011,13(2):138-143. |
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| 作者姓名： | 姜莉莉 顾春波 左一鸣 王开运 段海明 |
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| 作者单位： | 1.山东农业大学 植物保护学院,山东 泰安 271018 |
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| 基金项目： | 国家公益性行业(农业)专项 |
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| 摘 要： | 为明确抗多菌灵草莓枯萎病菌(ZY-R)菌株的生理生化特性,分析其可能的抗性机制,以课题组获得的抗多菌灵达53.91倍的ZY-R菌株及其敏感菌株(ZY)为研究材料,比较了ZY-R与ZY在苯丙氨酸解氨酶(PAL)和过氧化物酶(POD)活力及相对渗率等方面的差异。结果表明:以清水和不同浓度多菌灵处理两菌株1.5~24 h后,ZY-R体内的POD和PAL活力始终高于ZY的;敏、抗菌株间POD活力的差异远大于PAL。在处理时间内,PAL活力先上升后下降,6 h达到最高值;而POD活力一直处于上升状态,24 h达到最高值,且敏、抗菌株之间的差异达到最大,25 mg/L条件下ZY-R的POD活力为ZY的2.26倍;此外,与ZY相比,ZY-R可以渗出更多的电解质。
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| 关 键 词： | 草莓枯萎病菌 多菌灵 抗性 生理生化特性 |
| 收稿时间： | 9/7/2010 12:00:00 AM |
| 修稿时间： | 2010/10/17 0:00:00 |
Study on physiological and biochemical characteristics of carbendazim-resistant isolate of Fusarium oxysporum f.sp.fragariae ZY-R |
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| JIANG Li-li,GU Chun-bo,ZUO Yi-ming,WANG Kai-yun and DUAN Hai-ming.Study on physiological and biochemical characteristics of carbendazim-resistant isolate of Fusarium oxysporum f.sp.fragariae ZY-R[J].Chinese Journal of Pesticide Science,2011,13(2):138-143. |
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| Authors: | JIANG Li-li GU Chun-bo ZUO Yi-ming WANG Kai-yun and DUAN Hai-ming |
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| Institution: | 1.Department of Plant Protection,Shandong Agricultural University,Tai’an 271018,Shandong Province,China2.Zaozhuang Entry-Exit Inspection and Quarantine Bureau,Zaozhuang 277100,Shandong Province,China3.Taian Entry-Exit Inspection and Quarantine Bureau,Taian 271018,Shandong Province,China |
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| Abstract: | In order to find out the physiological and biochemical characteristics of Fusarium oxysporum f.sp.fragariae and analyze its resistant mechanism,the comparisons were conducted between the carbendazim-resistant isolate of F.oxysporum(ZY-R) and the sensitive isolate(ZY) on the activity of phenylalanine ammonia lyase(PAL) and peroxidase(POD),and the relative leakage that definite the physiological,biochemical characteristics of ZY-R.The results showed that the activity of POD and PAL of ZY-R was higher than tho... |
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| Keywords: | Fusarium oxysporum f sp fragariae carbendazim resistance physiology and biochemistry characteristics |
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